Answer the following questions pertaining to the experiment ofFigure 13.3.A. What would be the expected results if the Meselson and Stahlexperiment was carried out for four or five generations?B. What would be the expected results of the Meselson and Stahlexperiment after three generations if the mechanism of DNAreplication was dispersive?C. Considering the data from the experiment, explain why threedifferent bands (i.e., light, half-heavy, and heavy) can beobserved in the CsCl gradient. Experineatal levelConceptual levelL Add an excess of "N.oontainingcompounds to the growth mediumso all of the newby made DNA willcontain 4NGenerationsoltionAdd 14NSuspensionof bacterialcells labeledwith 1SN12 Incubate the cells forvaricus lengthsof time. Note: The 1SNJabeled DNAis shown in purple, and the 14NJabeledDNA is shown in blue00001 Lye the cells by adding byszymeand detergent, which disrupt thebacterial oell wall and cell37°CLywcellsUp to 4 generationsmembrane, respecthvely.DNACell wall4. Load a sample of the bysate onto aCCl gndient (Note The areragedensity of DNA is around 1.7 g/am?which is sufficie nty different from othercelular macromokcules.)LysateCagradientCell membrane5 Centrifiuge the gradients untilEquilibeium density centrifugationthe DNA molecules reach theirequilibrium densitiesLightDNAHalfbearyDNA6 DNA within the gradient can beobserved under a UV light.UVHeavylightDNA(Result shown here isafter 2 generations.)११११११

DNA is the genetic material in most living organisms. It is the information hub of the cell that…

Answered: Experineatal level Conceptual level L…

Biology 2e

2nd Edition

ISBN:9781947172517

Author:Matthew Douglas, Jung Choi, Mary Ann Clark

Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark

Chapter23: Protists

Section: Chapter Questions

Problem 26CTQ: Giaidia iamblia is a cyst-forming protist parasite that causes diarrhea if ingested. Given this...

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Answer the following questions pertaining to the experiment of
Figure 13.3.
A. What would be the expected results if the Meselson and Stahl
experiment was carried out for four or five generations?
B. What would be the expected results of the Meselson and Stahl
experiment after three generations if the mechanism of DNA
replication was dispersive?
C. Considering the data from the experiment, explain why three
different bands (i.e., light, half-heavy, and heavy) can be
observed in the CsCl gradient.

Experineatal level
Conceptual level
L Add an excess of "N.oontaining
compounds to the growth medium
so all of the newby made DNA will
contain 4N
Generation
soltion
Add 14N
Suspension
of bacterial
cells labeled
with 1SN
1
2 Incubate the cells forvaricus lengths
of time. Note: The 1SNJabeled DNA
is shown in purple, and the 14NJabeled
DNA is shown in blue
0000
1 Lye the cells by adding byszyme
and detergent, which disrupt the
bacterial oell wall and cell
37°C
Lyw
cells
Up to 4 generations
membrane, respecthvely.
DNA
Cell wall
4. Load a sample of the bysate onto a
CCl gndient (Note The arerage
density of DNA is around 1.7 g/am?
which is sufficie nty different from other
celular macromokcules.)
Lysate
Ca
gradient
Cell membrane
5 Centrifiuge the gradients until
Equilibeium density centrifugation
the DNA molecules reach their
equilibrium densities
Light
DNA
Halfbeary
DNA
6 DNA within the gradient can be
observed under a UV light.
UV
Heavy
light
DNA
(Result shown here is
after 2 generations.)
११११११

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