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Explain how to conduct a Kirby-Bauer disk diffusion test. Include all of the steps involved.
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- Discuss the medical application of the Benedict’s test? What other test(s) are used in parallel to Benedict’s test?Describe the Ninhydrin test. What does Ninhydrin test detect?Discuss in chemical detail the mechanism of action (how it works) of IMAC. Compare and contrast gel filtration chromotography with IMAC. Discuss the pros and cons for each technique. Why would a scientist prefer one technique over another? In what situations would one technique be more viable than the other?
- The following are errors that people commonly make when they perform serial dilutions. Indicate whether you think that the number of cfu/ ml calculated would be too high or too low if you make this mistake. You intend to add 0.9 ml of diluent to each tube and 0.1 ml of culture. Instead, you add 0.5 ml of diluent to each tube and 0.1 ml of culture to the first tube. Then, you make a serial dilution of 0.1 ml into and from each tube as described. You prepare 0.9 ml of diluents in each tube. You add 0.1 ml of culture (from the overnight culture provided) to every tube. You add 0.9 ml of diluent to each tube. You add 0.1 ml of culture to the first tube and mix. You get distracted, and transfer 0.1 ml to the third tube instead of the second. You perform the rest of the series as described.Explain how the Kirby-Bauer test works and what information it provides. Define minimum inhibitory concentration (MIC). Describe how the E-test works and what information it provides.Discuss the principles and applications of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry in molecular diagnostics.
- During a mass spectrometry experiment, the spectrum of vascular endothelial growth factor A (VEGF-A) was acquired. The absolute error of VEGF-A, having a mass of 1024.5210 Da was 0.06 Da. Calculate the relative error (ppm).How is 10^4 derived? Please show all calculation steps. # of cells per mL = (Average)*DF*10^4For the serial dilution, your stock solution must have a concentration of 3.5 mg/mL. How much diluent must be added to the 5.3 mg/mL red cell to prepare the stock solution? Show pertinent solution/s. What are the initial concentrations used for tubes 3, 5, and 6? Show pertinent solutions. What are the dilutions of the last positive tube and first negative tube respectively? Show computation.
- Compare and contrast the method for determining the minimum inhibitory concentration (MIC) with the Kirby-Bauer disc diffusion test.After blood collection, the red cells are separated from the serum to be used for the preparation of the stock solution. How is it done? For the serial dilution, your stock solution must have a concentration of 3.5 mg/mL. How much diluent must be added to the 5.3 mg/mL red cell to prepare the stock solution? Show pertinent solution/s. What is the appropriate diluent used for the preparation of the red cell suspension?A flow cytometer is constructed of many components. For good quantitative results how and how frequently should the various systems be calibrated? What is MESF and how does it apply in flow cytometry?