Explain why the maximum initial reaction rate cannot be reached at low substrate concentrations
Q: For the following aspartate reaction in the presence of inhibitor, Km = 0.00065 M. Determine Vmax in...
A: Enzymes are usually protein molecules which catalyzes biochemical reaction by decreasing the activat...
Q: In glycolysis, the conversion of phosphoenolpyruvate (PEP) to pyruvate is considered irreversible. Y...
A: In glycolysis there are three highly exergonic steps that are also regulatory irreversible steps whi...
Q: 2. Indicate whether each of the following statements describes a reversible competitive inhibito rev...
A: Enzymes are usually protein molecules which catalyzes the biochemical reaction by decreasing its act...
Q: 9. As increased intracellular calcium is detrimen- tal to the cell, calcium homeostasis is tightly r...
A: Apoptosis is programmed cell death. Whenever a cell fails to carry out its normal functioning, it is...
Q: UDP , gemcitabine was shown to undergo two successive phosphorylations at . The product, gemcitabine...
A: GEMCITABINE is a chemotherapy medication, used to treat cancer such as breast cancer, testicular can...
Q: Which is the correct sequence? 1. phospholipase activated 2. G protein activated 3. PIP2 split 4. pr...
A: Events are given for the signal transduction pathway which leads to protein phosphorylation
Q: Tabulate the different protein precipitation methods by completing the table below.
A: There are different methods of protein precipitation like organic solvent precipitation, ammonium su...
Q: HN-CO- HO- HO, HO ÇI HO- Но- HO- NHAC H H N „NH2 N H `N' H HN. HOOC Но Он ÓH HO OH -OH OH OH Teicopl...
A: It is a glycopeptide antibiotic produced by Actinoplanes teichomyceticus. It is made up of five majo...
Q: Explain what is meant by “stereospecificity.”
A: Specificity is the key term in biochemistry mainly in the study of enzymes. It is the ability of an ...
Q: During vigorous exercise, a buffer system consisting of lactic acid and sodium lactate is produced i...
A: Given Values: pKa of lactic acid = 4.76 The concentration of lactic acid in muscle cells = 1.5 μM Th...
Q: Explain the importance of adding TE Buffer as the final solution to isolate the concentrated DNA.
A: DNA isolation is a process of extracting nuclear material from a tissue sample. The tissue sample is...
Q: Select the term in column B which best matches the description in column A. There are more terms the...
A: Since we only answer up to 3 sub-parts, we’ll answer the first 3. Please resubmit the question and s...
Q: Why are buffers important in biochemical experiments? Please explainw ell
A: Buffer : solution which resists any change in pH via the addition of any acidic/ the basic component...
Q: What is a virus and how does the immune system kill viruses? What are the 5 examples of food that ca...
A: The science that deals with the study of viruses is called virology. Viruses are classified based on...
Q: Describe the methods used to identify the location of DNA binding proteins in the regulatory regions...
A: DNA binding sites are a form of binding website online determined in DNA wherein other molecules may...
Q: 1. Calculate Vmax and Km???
A: Enzymes are usually a protein molecules that catalyzes the biochemical reactions without being consu...
Q: 8. Maltose is a reducing sugar because A. It is a monosaccharide B. It can be reduced C. It is a pen...
A: Carbohydrates are also known as hydrates of carbon. They contain elements such as carbon, hydrogen a...
Q: Use the Stokes-Einstein equation to estimate the diffusion coefficient in m2/s and in um?/s for the ...
A: The Stoke-Einstein equation is used for calculating the diffusion coefficient of the spherical molec...
Q: Balance the chemical equation for the oxidation of arachidonic acid to ATP, CO2, and water. (Use the...
A: In the question given, Arachidonic acid is oxidized to ATP CO2 and water. Fatty acid oxidation proce...
Q: 3. What is the name of the molecule in the circled region labelled M (be specific)? How do you know?...
A: The backbone of a nucleic acid is made up of a sugar-phosphate chain linked by phosphodiester linka...
Q: Please explain the theoretical background (positive color reactions and what is being detected) invo...
A: Proteins are the polymers of amino acids. The amino acids are joined together by the peptide bonds. ...
Q: Describe TWO (2) methods transport of solutes in the systems with the aid of diagram I want a clear...
A: Solute transport is an important physiological function of all living forms. Solute transport occurs...
Q: What is plant biochemistry?
A: Introduction - Plant Biochemistry is the study of different chemical reactions in the plant such as...
Q: Which of the following use hydrogen bonds in the body: 1. Water (high specific heat, resist changes ...
A: The chemical link in which a hydrogen atom is attracted to an electro-magnetic atom is known as a hy...
Q: In the structure what is the difference of vancomycin and teicoplanin?
A: Teicoplanin and vancomycin are natural compounds that belong to the glycopeptide antibiotic family o...
Q: Briefly describe the relationship between degree of unsaturation and the physical characteristics of...
A: The lipids are the substances that are soluble in organic solvents like chloroform and ether, insolu...
Q: Examine the diagram to the right to complete the following questions. 9. Which letter (a, b, s or d)...
A: Ionic bond-It is the bond form by the transfer of electron from one atom termed as donor to a accept...
Q: 3. What properties do all monosaccharides have?
A: Monosaccharides: Simplest carbohydrates(have single sugar molecule) example glucose, fructose.
Q: Voer die titrasie uit op die peptied Ala-Lys-His-Ala uit / Do the titration on the peptide Ala-Lys-H...
A: Here Ala-His-Lys-Ala is a tetra peptide consist of two alanine at two end, one His and One Lys. The ...
Q: What is the general name for a monosaccharides which has 6 carbons 5 carbons • 3 carbons
A: Carbohydrates are classified into three types on the basis of number of sugar units they contain whi...
Q: Temperature greatly influences the reaction rate of enzymes. Expound this statement and relate this ...
A: Enzymes are biological catalysts, that increase the rate of a chemical reaction. The factors affect...
Q: Hello, please directly answer the questions 1, 2, 3 and 4 1. Biological relationship between hydroge...
A: Enzymes are biological catalysts that are proteins. Chemical reactions are sped up with catalysts. S...
Q: In the first step of the citric acid cycle, an acetyl group from acetyl CoA reacts with oxaloacetate...
A: Citric acid cycle is one of the steps in aerobic oxidation of glucose. It occurs in mitochondrial ma...
Q: Is the Salkowski test a specific test for cholesterol? Justify your answer.
A: Cholesterol is a type of steroid found in animals. It is the major component of the cell membrane. ...
Q: Does Your PDB code: 3GRS have a quaternary structure? YES OR NO? https://www.rcsb.org
A: 3GRS is Glutathione Reductase which is an oxidoreductase (flavoenzyme).
Q: What are the 2 divisions of the immune system? and What are the 5 parts of the immune system?
A: The immune system is a complicated network of cells and proteins that protects the body from infecti...
Q: Vancomycin is made up of seven-membered peptide chain. What are they
A: Vancomycin is an antibiotic made up of a tricyclic ring and each tricyclic ring is composed of vanco...
Q: Which of the alternatives below indicates techniques used both to purify and to concentrate proteins...
A: Proteins are polymers of amino acids linked by peptide bond and it is one of the important biomolecu...
Q: How may genes play a role in the susceptibility of inheriting cancers? The reproductive system discu...
A: Cancer is a disease in which the body's cells grow out of control and spread to other tissues. Any o...
Q: OH HO HO A NH2 Но. HO R-O. HO. HO HO R-O, OH Но HO `OH OH Но R-O, D HO. но но HO Но `OH OH B но. `OH...
A: Glycosidic bond is a type of covalent bond formed after reaction between hydroxyl group of carbohydr...
Q: You have obtained experimental kinetic data for two versions of the same enzyme, a wild‑type and a m...
A: Michaelis-Mention constant (Km) or equilibrium constant for the dissociation of the E-S complex is e...
Q: DIRECTIONS: 1. On the cholesterol molecule, label the region that would be embedded in the membrane ...
A: Hi. Thank you for the question. As per the honor code, we are allowed to answer three sub-parts at a...
Q: What hormone directly opposes the actions ofnatriuretic hormones?a. reninb. nitric oxidec. dopamined...
A: Natriuretic hormones (NH) are compounds that act in an endocrine or paracrine fashion to modify extr...
Q: What is the major tissue source of Alanine Aminotransferase? 2. What is the clinical significance o...
A: Alanine Aminotransferase(ALT) Enzyme which catalyzes amino group tranfer to alpha-ketoglutarate from...
Q: Name three fish/fishery product-derived glycosaminoglycans (GAGs) and their important applications i...
A: Glycisaminoglycans ( GAGs) in marine animals are different to those of terrestrial organisation, mai...
Q: The G° of the reaction of reactant X to product Y is the same in its catalytic reaction and the unca...
A: Enzymes are protein molecules that catalyze the biological reactions inside the cell. Enzymes do not...
Q: Essay 2 Summary Response A X Xunw w du/courses/1118169/quizzes/3741087/take Question 7 Match the phr...
A: Match the phrase with the correct cell respiration substance. 10. Electron transport system...a. y...
Q: 25. Given the following mRNA codons and amino acids, construct a polypeptide from this DNA strand. D...
A: A codon is a trinucleotide sequence of nucleic acid that corresponds to a specific amino acid. The g...
Q: 11.The fatty acid with the IUPAC name of hecadecanoic acid is b. myristic acid. C. palmitic acid. d....
A: The answers are as follows:
Q: (A) Do electron transport chains create a gradient so that ATP synthase can generate ATP molecules? ...
A: ATP synthase is a mitochondrial enzyme machinery which is located in the inner membrane of mitochond...
1. Explain why the maximum initial reaction rate cannot be reached at low substrate concentrationsPredict or describe the absorbance or enzyme activity at:
-
- pH = 2
- pH = 14
Explain your predictions
2. Explain why the maximum initial reaction rate cannot be reached at low substrate concentrations.
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
- 11) Using the data above, construct Lineweaver-Burk plots. First, graph the inverse of the reaction velocity (rate of reaction) data in column II versus the inverse of the methanol concentration in column I. Then, on the same graph, plot the inverse of the reaction velocity (rate of reaction) data in column III versus the inverse of the methanol concentration in column I. The optimal conditions for salivary lysozyme (hydrolyzing glycoproteins of bacterial wall) are 37 C - temperature and pH is 5.2. Explain the decrease in this enzyme activity if the temperature will rise up to 60 °C and pH will be changed to 8.0. To answer the question: a) draw the graph of the velocity dependency on temperature and pH; b) calculate the relative enzyme activity if 10 mg of lysozyme catalyzes the formation of 5 uM of the product per 2 minutes. Concidor NH3: 5.An enzymatic reaction follows M-M kinetics with Vmax= 2.5 mol m-3s-1and Km = 5 mM.Calculate the time required for 50% conversion of the substrate in a batch reactor if theinitial substrate concentration is0.2 M.Show your calculation steps.
- 2. Enzyme-catalyzed reactions. Answer the following with true or false. If false, explain why. (a) The initial rate of an enzyme-catalyzed reaction is independent of substrate concentration. (b) At saturating levels of substrate, the rate of an enzyme-catalyzed reaction is proportional to the enzyme concentration. (c) The Michaelis constant Km equals the substrate concentration at which velocity (v) = Vmax/2. (d) The Km for a regulatory enzyme varies with enzyme concentration. (e) If enough substrate is added, the normal Vmax of an enzyme-catalyzed reaction can be attained even in the presence of a noncompetitive inhibitor. (f) The Km of some enzymes may be altered by the presence of metabolites structurally unrelated to the substrate. (g) The rate of an enzyme-catalyzed reaction in the presence of a rate-limiting concentration of substrate decreases with time. (h) The sigmoidal shape of the v versus [S] curve for some regulatory enzymes indicates that affinity of the enzyme for the…1. Explain what happens to the velocity in the following scenarios. a. More substrate is added when the substrate concentration is low. b. More substrate is added when the enzyme is saturated with substrate. 2. If an enzyme has a K = 9.00mM and V max = = 1.85mM s', what would the velocity be if you had a substrate concentration of 3.0 mM? b. What would happen to the velocity if K, decreased to 3.0mM? 3. A researcher recorded the following velocity (R) data with an enzyme whose substrate is ATP. Find V and K, and show work. CMI 4. How would each of the following effect the rate of an enzymatic reaction? a. Increase of temperature (from 0C-100C) b. Increase in pH c. Increase in substrate concentration d. Increase in enzyme concentrationConsider the Michaelis-Menten equation, below: Vmaz (S V. k + [S] %3D What is the relationship between changes in substrate concentration and velocity when the concentration of substrate, [S), is well below k7 The S terms cancel out in this equation, so there is no effect of changing substrate concentration. The S] term in the numerator is negligible, so there is no impact of changing substrate concentration. Because the enzyme has reached Vr there is no effect of changing substrate concentrations on enzyme velocity. OThe [S term in the denominator is negligible compared to k There is a linear relationship between substrate concentration and velocity.
- Give two advantages and two disadvantages to using the biuret reaction to measure protein concentration compared to measuring the protein absorbance directly at 280 nmSelect true if the statement is CORRECT and false if OTHERWISE 1. Enzymes are catalysts and increase the speed of a chemical reaction without themselves undergoing any permanent chemical change. 2. Catalysis is defined as the acceleration of a chemical reaction 3. if the amount of the enzyme is kept constant and the substrate concentration is then gradually increased, the reaction velocity will decrease. 4. In the Induced-fit Model, if a dissimilar substance which does not fit the site is present, the enzyme rejects it 5. The Michaelis constant Vo is defined as the substrate concentration at 1/2 the maximum velocity. 6. A prosthetic group - an organic substance which is dialyzable and thermostable which is firmly attached to the protein or apoenzyme portion. 7. The rate of an enzyme-catalyzed reaction increases as the temperature is raised beyond optimum temperature. 8. Enzymes can be classified by the kind of chemical reaction catalyzed. 9. The living cell is the site of tremendous…Please ASAP. Thank you. Explain these results in short answer form. Why was the slope zero for blank solution? What was the optimal concentration for peroxidase activity? Do these values make sense?
- a. From experimental data obtained in Figure 1, construct a plot and equation that determines the rate constant, kM, of the MOX activity in conditions A and B of growth culture of H. polymorpha on methanol. Justify the selection of plot and findings. b. From the data behaviour presented in Figure 1, describe the enzymatic activity of MOX. What factors may influence the enzymatic activity of MOX at different condition batch cultures of H. polymorpha.Explain as brief and simple as possible. Answers must not be more than 30 WORDS each. a. How does the induced-fit model of enzyme action explain the broad specificities of some enzymes? b. In competitive inhibition, can both the inhibitor and the substrate bind to an enzyme at the same time? Explain your answer c. Why is penicillin toxic to bacteria but not to higher organisms?16) The graph at right shows the results of reaction rate vs. substrate concentration for a Michaelis-Menten type enzyme a. True b. False 17) The equilibrium constant for the conversion of the disaccharide sucrose to the simple sugars glucose and fructose is 140.000 reaction rate->