Fill in the following blanks. As the dye concentration increases, the absorbance of the light       [linearly increases, linearly decreases, exponentially increases or exponentially decreases]. The dye concentration units are [molar (M), millimolar (mM) or grams per liter (g/L)]  . Although most people wrongly conclude that absorbance has units nm, in fact, absorbance is unitless. This is because it results from a ratio of light through a colored sample relative to light intensity through a clear (blank) sample.  The units for the slope of the graph would be       [mM, 1/mM or nm]  . Hint: the slope is the change in y values divided by the change in x values. The units for the y-intercept of the graph would be       [nm, nM or unitless]  . The value for the slope of the line that describes the absorbance data is       [6.4597 or 0.0008]  The value for the y-intercept of the line that describes the absorbance data is       [6.4597 or 0.0008]  .

Fundamentals Of Analytical Chemistry
9th Edition
ISBN:9781285640686
Author:Skoog
Publisher:Skoog
Chapter27: Molecular Fluorescence Spectroscopy
Section: Chapter Questions
Problem 27.9QAP
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Fill in the following blanks.

As the dye concentration increases, the absorbance of the light       [linearly increases, linearly decreases, exponentially increases or exponentially decreases].

The dye concentration units are [molar (M), millimolar (mM) or grams per liter (g/L)]  .

Although most people wrongly conclude that absorbance has units nm, in fact, absorbance is unitless. This is because it results from a ratio of light through a colored sample relative to light intensity through a clear (blank) sample. 

The units for the slope of the graph would be       [mM, 1/mM or nm]  . Hint: the slope is the change in y values divided by the change in x values.

The units for the y-intercept of the graph would be       [nm, nM or unitless]  .

The value for the slope of the line that describes the absorbance data is       [6.4597 or 0.0008

The value for the y-intercept of the line that describes the absorbance data is       [6.4597 or 0.0008]  .

B ROX
6.4 Electronic Struct X
courses/555285/quizzes/1691848/take
]0[
A solution standard is one where the experimenter has carefully prepared
the solution to know the concentration precisely.
Red #40 Dye
Concentration (mm)
A student prepares a variety of standard Red #40 solutions with the
following concentrations. She also uses a spectrophotometer to measure
the absorbance of 500 nm (green) light by those standard solutions. She
obtains the following data.
0.020
0.035
0.040
0.045
0.050
Quiz: Exp. VII - Pre- X
orbance
0.35
0.3
0.25
0.2
0.15
b Success Confirmatic X
Absorbance
0.13
0.24
0.25
0.30
0.32
She prepares a calibration curve (graph) of the data using Microsoft Excel
and fits the data to a linear equation.* She obtains the following graph:
Absorbance of Green Light by Red#40 Solutions
(2) ISEKAI D&D ✔"
x +
38°F Cloudy
=S
U
6:12 PM
3/20/2023
Transcribed Image Text:B ROX 6.4 Electronic Struct X courses/555285/quizzes/1691848/take ]0[ A solution standard is one where the experimenter has carefully prepared the solution to know the concentration precisely. Red #40 Dye Concentration (mm) A student prepares a variety of standard Red #40 solutions with the following concentrations. She also uses a spectrophotometer to measure the absorbance of 500 nm (green) light by those standard solutions. She obtains the following data. 0.020 0.035 0.040 0.045 0.050 Quiz: Exp. VII - Pre- X orbance 0.35 0.3 0.25 0.2 0.15 b Success Confirmatic X Absorbance 0.13 0.24 0.25 0.30 0.32 She prepares a calibration curve (graph) of the data using Microsoft Excel and fits the data to a linear equation.* She obtains the following graph: Absorbance of Green Light by Red#40 Solutions (2) ISEKAI D&D ✔" x + 38°F Cloudy =S U 6:12 PM 3/20/2023
B ROX
6.4 Electronic Struct X
courses/555285/quizzes/1691848/take
Concentration (MIVI)
]
0.020
0.035
0.040
0.045
0.050
Absorbance
She prepares a calibration curve (graph) of the data using Microsoft Excel
and fits the data to a linear equation.* She obtains the following graph:
0.35
0.3
0.25
0.2
0.15
0.1
0.05
0
Quiz: Exp. VII - Pre- X
0
Absorbance of Green Light by Red#40 Solutions
0.13
0.24
0.25
0.30
0.32
▬▬
Fill in the following blanks.
b Success Confirmatio ×
y = 6.4597x + 0.0008
R² = 0.9885
0.01
0.02
0.05
Red #40 Dye Concentration (mm)
0.03
0.04
0.06
(2) ISEKAI D&D ◄ x +
38°F Cloudy
= 0
U
6:12 PM
3/20/2023
:
Transcribed Image Text:B ROX 6.4 Electronic Struct X courses/555285/quizzes/1691848/take Concentration (MIVI) ] 0.020 0.035 0.040 0.045 0.050 Absorbance She prepares a calibration curve (graph) of the data using Microsoft Excel and fits the data to a linear equation.* She obtains the following graph: 0.35 0.3 0.25 0.2 0.15 0.1 0.05 0 Quiz: Exp. VII - Pre- X 0 Absorbance of Green Light by Red#40 Solutions 0.13 0.24 0.25 0.30 0.32 ▬▬ Fill in the following blanks. b Success Confirmatio × y = 6.4597x + 0.0008 R² = 0.9885 0.01 0.02 0.05 Red #40 Dye Concentration (mm) 0.03 0.04 0.06 (2) ISEKAI D&D ◄ x + 38°F Cloudy = 0 U 6:12 PM 3/20/2023 :
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Follow-up Question

a student in the previous question measured the absorbance of green light from a beverage that contains Red#40 and She finds that the absorbance of the beverage is 0.322. Using the calibration curve from the previous question, what would be the concentration (mM) of Red#40 in the beverage?

0.0322, 0.0497, 2.069, or 0.0008
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