For each wet mount, describe the appearance of the red blood cells, determine the solution's tonicity, and indicate the net water movement relative to the cell (into the cell, no net movement, out of the cell). Tube (NaCl) Appearance of RBCs Tonicity of Solution Net Water Movement 10 g/dl 3.5 g/dl 0.85 g/dl 0.45 g/dl 0.20 g/dl
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- Suppose like :- At 10 am you received a doctors order that you have to Infuse 1,000ml of PNSS in 10 hours to Mr Dela Cruz. What is the correct rate of this isotonic solution when the drop factor is 10 gtt = 1ml ? 15.73 qtts/min 31.249 qtts/min 49 qtts/min 18 qtts/min.At 10 am you received a doctors order that you have to Infuse 1,000ml of PNSS in 10 hours to Mr. Dela Cruz. What is the correct rate of this isotonic solution when the drop factor is 10 gtt = 1ml? 15.73 qtts/min- 31.249 qtts/min 16.67 qtts/min 18 qtts/minYou have a solution of 0.30 M NaCl. NaCl is a non-penetrating molecule. If you place red blood cells into this solution predict what would happen to the red blood cells (stay same, crenated, lysed). Determine: 1. the osmolarity of 0.30 M NaCl. 2. apperance/effect on RBC 3. tonicity
- Osmotic experiment is done using equivalent dilutions of 2% urea. Note the erythrocyte membrane permeability constants (P) for urea, sodium (Na+) and chloride ions (Cl-) are 152, 0.25 and 0.1 x 10-4 cm.s-1, respectively. Select all of the following which apply. The permeability constants indicate that red blood cell membranes are much more permeable to urea, thus urea will enter the cells (a permeating solute) and exerts an osmotic effect with water leaving the cells. 1% NaCl is equivalent to 2% urea, because NaCl dissociates into Na+ and Cl-, thus resulting in a solution of the same osmolarity as the 2% urea, which does not dissociate. The permeability constants indicate that red blood cell membranes are much more permeable to urea, thus urea will enter the cells (a permeating solute) and does not exert an osmotic effect. The permeability constants indicate that red blood cell membranes are much less permeable to urea, thus urea will not enter the cells (a…A. You have 3% (w/v) concentrated saline in 20 mL ampoules. What volume and how many ampoules do you need to make 1 L of an isotonic saline solution (0.9% w/v). i) 300 mL of 3% (w/v) saline; 15 ampoules 360 mL of 3% (w/v) saline; 18 ampoules 120 mL of 3% (w/v) saline; 6 ampoules 240 mL of 3% (w/v) saline; 12 ampoules iii) iv)You have a solution of 0.30 M glycerin. Glycerin is a lipid molecule and penetrates through the cell membrane. If you place red blood cells into this solution predict what would happen to the red blood cells (stay same, crenated, lysed). Determine: 1. the osmolarity of 0.30 M glycerin 2. apperance/effect on RBC 3. tonicity
- calculate the drop rate of an IV if 1000mL of a 5% glucose solution is to be administered over a period of a 8h and the drop factor is 20 gtt/mL20 mg active ingredient in 200ml normal saline. What is the weight/ volume concentration?A. You have 3% (w/v) concentrated saline in 20 mL ampoules. What volume and how many ampoules do you need to make 1 L of an isotonic saline solution (0.9% w/v). i) 300 mL of 3% (w/v) saline; 15 ampoules iii) 360 mL of 3% (w/v) saline; 18 ampoules 120 mL of 3% (w/v) saline; 6 ampoules 240 mL of 3% (w/v) saline; 12 ampoules iv) B. A hypothetical organism, 'Macquarie biomolecula' is an anaerobic thermophilic microbe that is found in hydrothermal vents. It lives at temperatures near the boiling point of water and at pressures over 200 times higher than at sea level. It does not contain peptidoglycan. This organism is a type of: i) Eukarya ii) Bacteria iii) Archaea iv) Protista c. Which of the following statements is correct? i) all RNA viruses are single-stranded, and all DNA viruses are double-stranded ii) a virus is a type of cell iii) a virus infects a single host species iv) retroviruses contain RNA as their genome D. Which of the following may be found in the circulation? i)…
- Calculate the elution volume of blue dextran (blue), myoglobin (red), and Bromocresol purple (purple) in the gel filtration chromatography below with a flow rate of 2.5 mL/min. (Take note that the black colored test tubes represent colorless eluents)A. a pre-weighted dialysis bag which contained a solution of 10% glucose was placed in a beaker which contained 20% glucose. After one hour, the bag was weighted again. Calculate the % mass change of the dialysis bag from the following information: Mass of bag before the experiment: 15.3g Mass of bag after the experiment: 12.7% B. was the beaker solution hypertonic, hypotonic, or isotonic?Transport of biomolecules to tissues takes place through capillary walls by both convection and diffusion. Consider an endothelium in which the gaps between the cells are characterized by the following dimensions: L=1 µm long, h=200 nm high, and W=10 nm in width (the last dimension is the distance between the two cells). The fluid is at 37degC and has the same properties as physiologic saline. Let the average pressure in the capillary be 45 mm Hg and let the pressure in the tissue be 0 mm Hg (ignore osmotic effects). Consider a small solute that has a diffusion coefficient of 1x10-5 cm2/s. Does this solute pass across the endothelium by diffusion or is it primarily carried by flow?