Give at least two common loading control used in Western Blotting?
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Give at least two common loading control used in Western Blotting?
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- For the hybridization step in the Southern blotting, the hybridization was done at 85 degrees Celsius instead of 58 degree Celsius. Explain how this would affect my results.1) Enhanced GFP protein has: An excitation peak at 488nm and emits maximally at 507nm. An excitation peak at 488nm and emits maximally at 587nm An excitation peak at 488nm and emits maximally at 567nm. An excitation peak at 458nm and emits maximally at 507nm. 2) A typical sequencing read will yield _____ nucleotides of unambiguous sequence. 1800-2000 3800-4000 2800-3000 800-1000 3) Sequencing primers should be designed to bind where? ~ 25 nucleotides upstream of the beginning region to be sequenced. ~ 25 nucleotides downstream of the beginning region to be sequenced ~ 60 nucleotides upstream of the beginning region to be sequenced. ~ 60 nucleotides downstream of the beginning region to be sequencedWhat do you mean by Southern Blotting technique?
- For the CPT code assignment 44970, which of the following coding references can be accessed from the coding summary screen? Select all that apply.Why are there multiple results returned when you input a sequence into BLAT or BLAST? How do you know which is most relevant? Does that make the others irrelevant?Discuss which barcodes to use for bacteria, animals, plants and fungi and why with references (1000 words)
