Given a 0.100 M solution of anserine at its isoelectric point and ready access to 0.200 M HCl, 0.200 M NaOH and distilled water, describe the preparation of 1 L of 0.0300 M anserine buffered solution, pH 7.20.   Volume of stock solution needed = ? mL   Volume of HCl needed to adjust the imidazole group = ? mL   Volume of HCl needed to titrate the amino group = ? mL   Total HCl needed = ? mL

Chemical Principles in the Laboratory
11th Edition
ISBN:9781305264434
Author:Emil Slowinski, Wayne C. Wolsey, Robert Rossi
Publisher:Emil Slowinski, Wayne C. Wolsey, Robert Rossi
Chapter24: The Standardization Of A Basic Solution And The Determination Of The Molar Mass Of An Acid
Section: Chapter Questions
Problem 2ASA: In an acid-base titration, 21.16 mL of an NaOH solution are needed to neutralize 20.04 mL of a...
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Given a 0.100 M solution of anserine at its isoelectric point and ready access to 0.200 M HCl, 0.200 M NaOH and distilled water, describe the preparation of 1 L of 0.0300 M anserine buffered solution, pH 7.20.

 

Volume of stock solution needed = ? mL

 

Volume of HCl needed to adjust the imidazole group = ? mL

 

Volume of HCl needed to titrate the amino group = ? mL

 

Total HCl needed = ? mL

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