Given a 0.300 M solution of anserine at its isoelectric point and ready access to 0.300 M HCl, 0.300 M NaOH and distilled water, describe the preparation of 1 L of 0.0500 M anserine buffered solution, pH 7.20. Volume of stock solution needed =___ mL Volume of HCl needed to adjust the imidazole group = ___ mL Volume of HCl needed to titrate the amino group = ___mL Total HCl needed = ___ mL.

Fundamentals Of Analytical Chemistry
9th Edition
ISBN:9781285640686
Author:Skoog
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Chapter24: Introduction To Spectrochemical Methods
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Problem 24.1QAP
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Given a 0.300 M solution of anserine at its isoelectric point and ready access to 0.300 M HCl, 0.300 M NaOH and distilled water, describe the preparation of 1 L of 0.0500 M anserine buffered solution, pH 7.20.

Volume of stock solution needed =___ mL

Volume of HCl needed to adjust the imidazole group = ___ mL

Volume of HCl needed to titrate the amino group = ___mL

Total HCl needed = ___ mL.

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