Gradient elution used in HPLC. How is the gradient created for a reverse-phase HPLC experiment over the course of the run? A)Increase the pH of the mobile phase. B)Decrease the pH of the mobile phase. C)Increase the vapor pressure of the analytes by increasing the temperature. D)Increase mobile phase polarity by increasing the fraction of polar solvent in a binary mobile phase. E)Decrease mobile phase polarity by mixing with a less polar solvent to better compete with the nonpolar stationaryphase.

Gradient elution used in HPLC. How is the gradient created for a reverse-phase HPLC experiment over the course of the run? A)Increase the pH of the mobile phase. B)Decrease the pH of the mobile phase. C)Increase the vapor pressure of the analytes by increasing the temperature. D)Increase mobile phase polarity by increasing the fraction of polar solvent in a binary mobile phase. E)Decrease mobile phase polarity by mixing with a less polar solvent to better compete with the nonpolar stationaryphase.

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter33: Automated Methods Of Analysis

Section: Chapter Questions

Problem 33.5QAP: Give the advantages and disadvantages of sequential injection analyzers compared to traditional flow...

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Give the advantages and disadvantages of sequential injection analyzers compared to traditional flow Injection analyzers.
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