How can a suspect bacterium be fingerprinted?

Q: What is an example of an experimental approach for determining whether a beef burger contains a food…

A: Beef burgers served rare or undercooked may contain harmful bacteria that could cause food…

Q: Is  Bacillus cereus negative or positive for methyl red, and  Voges-Proskauer tests?

A: The methyl red (MR) test detects whether bacteria can ferment the glucose or not. It detects the…

Q: What is a mixed culture? A contaminated culture?

A: A microbiological culture, or microbial culture, is a technique for increasing microbial creatures…

Q: What is ORF scanning?

A: The open reading frame(ORF) in molecular genetics is a continuous stretch of codons that begins with…

Q: Why does a streaking method used to inoculate plates result in isolated colonies?

A: Streaking method is used to obtain pure colonies of microbes(bacteria) from a proper single species…

Q: Outline how samples are processed in the Diagnosis Bacterial Laboratory

A: Introduction: A dichotomous key is a tool used to identify natural entities such as microorganisms,…

Q: Transillumination is used clinically for the detection of:

A: Transillumination is an examination that is done with the help of a strong light shining on a…

Q: What is the methods used for identifying unknown bacteria?

A: Ability of identifying various microorganisms have many important application.For example,in the…

Q: Why is pure culture and culture collection important in identifying novel species of bacteria?

A: A pure culture is a population of cells or multicellular organisms growing in the absence of other…

Q: Why each bacterium must be tested separately?

A: Antibiotics are chemical substances that kill or inhibit or prevent the growth of microbes. They are…

Q: What order should the steps be in for this culture method?

A: A conditional mutant is a mutant that exhibits a wild-type phenotype under permissive conditions but…

Q: What is the difference between streak plate method and spread plate technique?

A: Answer of the question given below...

Q: What is the procedure of beta method?

A: Beta-lactamase test is used for the detection of enzyme beta-lactamase. This enzyme provides the…

Q: What is the significance of properly labeling collected specimen?

A: The specimen collected should be labelled properly before bringing it to the laboratory for testing.…

Q: What is the focus of the Housemann-Rodrik-Velasco growth diagnostic framework?

A: This growth diagnostic framework was give by Hausmann-Rodrik-Velasco.Framework suggests an…

Q: What is the purpose of making a serial dilution?

A: Given: In microbiology, serial dilutions (log dilutions) are used to reduce the bacterial…

Q: What is the importance of using a test/control organism in describing the cultural characteristics…

A: The food materials on which the organism is developed is called culture medium and the development…

Q: How can a suspect bacterium be fingerprinted?

A: DNA fingerprinting is a technique for detecting a high number of minisatellites in the genome at the…

Q: You are tasked to isolate a certain species of bacteria from a mixed culture to further characterize…

A: Bacteria are small single-celled organisms. Bacteria are found almost everywhere on Earth and are…

Q: Why does fixation of the positive dye help bacteria stick to the slides?

A: Dyes in microbiology are for staining cells; they are used based on the chemical properties and the…

Q: What is the biological and non-biological samples used in forensic DNA investigations?

A: Chromosomes are carrier of deoxyribonucleic acid (DNA). DNA is the genetic material. Each cell has…

Q: Describe three problems associated with using the standard plate count method for determining the…

A: Typically, an accurate count of bacterial colonies is required for biological processes. Microbial…

Q: What biochemical tests did we use to distinguish staphylococci from streptococci?

A: Staphylococcus and streptococcus both occur in cocci shape and both are gram positive organisms.…

Q: What are the principles of Chelex extraction and solid-phase DNA extraction methods?

A: To explain the principles of Chelex extraction and solid-phase DNA extraction methods The principle…

Q: If the bacterium was lysogenized by a bacteriophage that has a virulence gene, determine the kind of…

A: The phenotypic approaches involve identifying bacteria by taking into account their behaviour and…

Q: If you did the multiple-tube technique, list one advantage and one disadvantage of each method of…

A: Water from various sources obtained for routine testing were analyzed by Swedish standard methods…

Q: What are the key points necessary for proper collection ofclinical specimens, and why is it…

A: Clinical specimens are those biological specimens taken from a patient's body that will be used for…

Q: how to find out the test is differential or selective in the microbiology lab test?

A: Many kinds of medias are used in microbial study. Two types of media having similar inferred names…

Q: How accurate are DNA swab tests?

A: DNA swab tests for certain genetic variants.

Q: Explain why culture based biochemical and genetic testing would not be the ideal method to identify…

A: Microbial culture or microbiological culture is a technique in microbiology in which microorganisms…

Q: How do we reconcile the number of colonies in the plates to the actual number of bacteria in the…

A: Microbes may enter the body in a variety of ways and cause infection everywhere, but antigen and…

Q: How will you check the purity of an isolate?

A: Purity is defined as the absence of impurities in substance or the presence of matter other than…

Q: What is the Fluorochrome staining technique for Mycobacterium

A: Mycobacterium - they are a type of germ. There are different kind of mycobacterium. Still others…

Q: Why does RNA give a positive result to Biuret's test?

A: Biuret test is a chemical test used for detecting the presence of peptide bonds. In presence of…

Q: Why is it necessary to cut thin sections of the tissue sample from a specimen using a microtome?

A: Microtomes are specialized cutting instruments that produce very thin slices of tissues so that the…

Q: If zn stain is positive from a sputum sample, what would be your pre-treatment of choice to isolate…

A: Zn stain test also called as Ziehl-Neelsen test is a rapid method to detect the acid fast bacteria.

Q: Why should freshly collected samples be used for microscopic examination?

A: The benefit of the microscope is that it produces a better resolution than the naked eye. The higher…

Q: How can the thickness of the smear affect the staining procedure?

A: Smear can e defined as the spread of cells in a layer to observe it under microscope. Since, cells…

Q: In RPR test, Why is it necessary to rotate the slide/card?

A: RPR stands for Rapid Plasma Reagin. It is done to detect syphilis.

Q: Does the gram staining method still have a place in today's world of advanced diagnostic methods? If…

A: Gram staining is a type of staining which is used to differentiate bacteria on the basis of…

Q: What technique is used to stain bacteria? Who developed this technique?

A: Microorganisms are such small living organisms that are less than 0.1 mm, and can be seen only under…

Q: Why do DNA polymerases fromthermophilic microbes significantly improve the PCRprocedure?

A: Step 1 :Introduction PCR has been used widely in fields such as forensics, genetic testing, and…

Q: What is the purpose of using the streak plate method?

A: Solution-  Introduction -  Streak plate technique is used  for the isolation into a pure culture of…

Q: What is the goal of isolation streak plate technique? Why is microbial considered a pure culture?

A: Streaking : Streaking for isolation on an agar plate involves the successive dilution of organisms…