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How else could you test for purity of the phycocyanin sample? Briefly outline the steps to be taken
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- Gel used in electrophoresis is/are: a. Agarose gel b. Polyacrylamide plain gel c. Polyacrylamide SDS impregnated Polyacrylamide gel (Sodium dodecyl sulphate)We have used SAR as an estimate of purity. How else could you test for purity of the phycocyanin sample? Briefly outline the steps to be takenDiscuss the correct laboratory technique in using the centrifuge for qualitative analysis. Give two tips on its proper use. Explain how calcium ions are confirmed present in a solid salt by using flame test. Discuss a method to confirm the presence of a) phenol and b) ketone in a test compound. How are proteins confirmed present in a sample? Explain the laboratory process.
- Provide the Experimental procedure of the following: Example: Ninhydrin Test -The samples needed in this test were 1% casein solution, albumin and gelatin and the reagent used was ninhydrin (Triketohydrindenehydrate or Triketohydrin hydrate). Test tube, test tube stand, pippete, water bath, and spectrophotometer were the materials and equipment used. Ninhydrin reagent in 1-2 drops was added to 0.5 ml of the sample with care. It was mixed and heated to boiling for 1-2 minutes. Then, it was allowed to cool to record the observation. Test that needs Experimental procedure: 1. Heat denaturation Samples: Albumin, gelatin 2. Isoelectric Precipitation Samples: Casein 3. Concentrated Mineral Acids Samples: Albumin 4. Organic Solvents Samples: AlbuminFollowing electrophoresis of the two pGlo mini-preparation samples (both digested and undigested), the following agarose gel image was obtained. provide an accurate and detailed figure, describing the image and what it depicts.Discuss sabin feldman dye test.
- Write the protocol for lactophenol blue stain method indicating; materials, reagents, Principle, procedure and results.What are the mechanisms of samples separation work in Thin layer Chromatography? Please shortly write at your own words. Answer should be to the point (5-6 lines maximum).Why is it important that the agarose is exposed to ethidium bromide, which is either added to the gel itself or mixed to the staining solution? Explain the principle behind its use.
- On a paper, draw a schematic diagram on the Extraction of Flavonoids using the procedures stated below. Procedure: Extraction of Flavonoids Weigh 10 g of the shade dried plant sample and chop into small pieces. Macerate with 200 mL 70% ethanol for 3 days at room temperature (25°C). Filter the extracts in vacuum using Whatman filter. Evaporate to dryness. Weigh the residue and compute the percentage yield.In outline form discuss the steps of Ziehl-Neelsen staining Complete the table for National Reporting Scale (Description) National Reporting Scale Result Description 0 +n 1+ 2+ 3+What chemicals are used for the Simple Stain from our lab experience? Check all that apply. carbolic acid mycolic acid phenol acid fuschin malachite green safranin nigrosin iodine ethanol methylene blue crystal violet carbol fuschin Congo red acid-alcohol O O O O O O O O O O O O O O