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- How does the luciferase plasmid bind to estrogen receptors?What is the role of the Ti plasmid in plant tumor formation?Three haploid fungal mutants that require compound W for growth were isolated. Each mutant contains a recessive allele in a single gene. Three compounds (A, B and C) in the biosynthetic pathway to W are known, but their order in the pathway is unknown. Each compound is tested for its ability to support the growth of each of the three mutants. Phenotypes of all of the three mutants are shown in the following table (“+" indicates growth, "-" indicates no growth). A C W Mutant 1 Mutant 2 Mutant 3 What would be the phenotype of a haploid mutant that contains both mutant alleles in mutant 2 and 3? Phenotype refers to growth or absence of growth on compounds A, B, C and WN. O Like mutant 1 O Like mutant 2 Like mutant 3 O Like wild type
- Draw the Western Blot that would result from undertaking the same experiment in the presence of: i) An estrogen receptor agonist ii) An estrogen receptor antagonist iii) An estrogen receptor agonist in the presence of an excess amount of antagonist iv) Estrogen in the presence of an excess amount of antagonist v) An estrogen receptor inverse agonist.What is the role of X-gal? What color will cells with the plasmid be in the absence of X-gal? Why?The figure below shows the life cycle of the fungus Neurospora. The adult stage of the Neurospora is a multicellular haploid. b) Neurospora has an arginine amino acid synthesis pathway shown below. Suppose I take the strain above that only grows with arginine supplements and cross it to a different mutant Neurospora strain that grows with arginine and citrulline supplements but not with ornithine supplements. Assuming gens A, B, and C are unlinked and there is only one mutation per stain: What percentage of the progeny will grow on ornithine? What percentage on citrulline? What percentage on arginine?
- Give an example of a nucleoside analogue that has been used successfully as an antiretroviral drug. How does it work thanksWould the application of paclitaxel to a cell make us incapable of performing karyotyping on that cell? Why or why not?1)What is plasmid? 2) How do you explain the selection of plasmid for carrying the desired gene? 3) Follow the steps of human insulin production 4) Make a diagram for the production of growth hormone
- Choose the statement below that is true about the characteristics of the bacterial fertility factor factor. The fertility factor carries genes responsible for uptake of naked DNA, and has several IS elements to assist plasmid integration into host cell's chromosome. O The fertility factor carries genes responsible for formation of bacteriophages, and has several IS elements to assist plasmid transfer through the sex pilus. The fertlity factor carries genes responsible for formation of sex pili, and has several IS elements to assist plasmid integration into host cell's chromosome. The fertility factor carries genes responsible for formation of sex pili, and has several IS elements to assist plasmid transfer through the sex pilus.Why are bacterial DNA gyrase and topoisomerase good targets for antimicrobial drugs? These enzymes are not necessary for DNA replication. These enzymes are involved in energy production, so targeting them would prevent the bacteria from obtaining necessary energy. These enzymes are different from their eukaryotic counterparts, so the drugs targeting them should not affect the host. These enzymes are responsible for host cell recognition, so targeting them would prevent the host cell from being infected.Flag this QuestionQuestion 10Briefly describe the effects of colchicine treatment on cells. What are the genetic implications of such effects?