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- 1. An image of a DNA profile is shown below. The size marker fragments are not shown, but the size in bp is displayed along the top. 100 150 200 250 300 350 D8 Identifiler TM k 2000 multiplex STR TH01 1500 D13 D19 D5 VWA ТРОХ D16 CSF AMEL D3 D21 FGA 1000 D18 D7 D2 500 How do the multiplex STR data demonstrate the profile is from a man? Which loci are homozygous? How do you know this? How does the IdentifierTM kit differentiate D19 and D3 PCR products? How does the IdentifierTM kit differentiate D5 and FGA PCR products?2. Name the first complementary base from the 3' direction. Type your answer in ALL CAPITAL LETTERS. D OCH, -OCH2. The picture shows a segment of DNA from a cat. Which of these is most likely the kitten of this cat? CIRCLE YOUR ANSWER. а. 1 DNA Fingerprints b. 2 с. 3 d. 4
- The diagram below illustrates the results of electrophoresis of DNA sequences obtained from a family of two adults and three children, and amplified using PCR. The bands represent short repeating sequences of variable length. Results for another female (X) are included for comparison. Father Mother Child Child Child X 1 2 3 Origin―4. Practice three Below is the STR site of an individual. Could you list which loci are homozygous for the tested person? DNA Profile of Sample from Hotel Fire Victim Number of base pairs 225 250 275 100 125 150 175 200 300 325 350 375 400 D18551 D3S1358 TH01 D21S11 14 15 11 DSS818 DSS818 D13S317 07s820 0165539 18 10 9 11 WWA WA D8S1179 CSFIPO 15 15 AMEL ТРОХ ТРОХ FGA FGA 16.2 Standard Relative fluorescence7. A burned body is found after a warehouse fire. You cannot obtain nuclear DNA suitable for testing, but you are able to obtain a sample of the person's mtDNA. What challenge will you encounter when trying to identify the person using mtDNA? Everyone has the same mtDNA so you won't be able to narrow identity down. mtDNA is most useful for information on traits like hair color so it won't narrow things down much. mtDNA only shows patrilineal lineage so it will only show the father's mtDNA. mtDNA is time consuming and expensive; not all labs have the capabilities to test this.
- 1. Suppose that the three samples are from two parents and their child. Which individuals are the parents? Which individual is the child? How do you know? Parent 1 = Parents 2 = Child = 2. What genotype must each individual have for this scenario to be possible? Parent 1 = Parent 2 = Child = please answer all of them now.3. What are the genotypes possible for a person who has: A blood? B blood? O blood? AB blood? 4. A man with type AB blood is married to a woman also with type AB blood. Show the cross. What proportion of their children will have: A blood? B blood? O blood? AB blood? 5. A man has type B blood (genotype I³I®) is married to a woman with type O (ii) blood. Show the cross. What proportion of their children will have: 1. A blood? 2. B blood? 3. O blood? 4. AB blood? 6. A woman with type A blood (genotype I^i) is married to a type B person (genotype Iºi). Show the cross. What proportion of their children will have: A blood? B blood? О blood? AB blood?5. What would have happened if the gel were placed backwards in the electrophoresis chamber with the wells containing the DNA near the positive end of the gel? smiT 3 vea deJ ba ovitsgo- in Suspect #1's sample? ellow- 6b. How many DNA fragments were in Suspect #2°s sample? 6a. How many NA fragments 7. Suspect #1 and Suspect #2 have different numbers of fragments. Explain why DNA samples from different people can have different numbers of fragments. bn ovitieo+ 8. Explain the difference between tracking dye and DNA stain.
- Worksheet for Lab 12: DNA FINGERPRINTING Name Lab Day & Time - Negative End Marker Crime Suspect Suspect Standard Scene # 1 # 2 wells to eadms d al ib mot + Positive End nian AMa hos yb 1. When you simply look at the banding patterns on the gel from the DNA fingerprint we ran in lab as seen above, which two profiles match? 2. Does the fact that one suspect's DNA fingerprint matches the DNA at the crime scene prove their guilt? Why or why not? 3. The DNA was originally placed in the wells of the gel near the negative end of the gel and fragments moved towards the positive end of the gel. What charge do DNA molecules have? Positive 4a. Where on the gel are the longest fragments found? de gel, on lectrode 4b. Where on the gel are the shortest fragments found? IX che samples is mixeu w the sample to sink to the bottom of the wen. ally followed as it moves down the gel. TE buffer is matched to the gef TO assure- - bottom of the wells with the micropipette tip. tips contaminated with DNA in…2 of 13 3. It could be used to identify the paternity of an individual. Application Of DNA fingerprinting 1. Look at the diagrams below and indicated if the child came from the father in question? Provide evidence for your reason. CENA M C 000000000 F $265595 M C sesscecome F 25000000000002 Search 2. Look at the diagrams below and indicated if the evidence came from the victim or suspect in 0 - 0 0 D4. Draw the number of fragments as well as their sizes as they would migrate on the electrophoresis gel. Write the size of the fragments on the square when you draw them. Ladder Sal I Hae II Eco R1 bp 50,000 24,000 20,000 16,000 10,000 9,000 8,000 7,000 6,000 5,000 4,000 3,000 2,000 1,000 DNA migration