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If you placed one copy of your gene into a tube and ran a PCR for 3 cycles, how many copies of your gene would you have at the end?
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- Choose the correct statements from the list below. There may be more than one correct statement. A) If you start with 2 DNA templates, after four rounds of PCR you'll have 32 copies B) PCR is useful in making millions or billions of copies of a gene so that it is present in a quantity large enough to study C) quantitative PCR is very similar to PCR, but fluorescent probes are added so that we can measure how much PCR product exists by examining how much the reaction fluoresces D) In real-time reverse transcriptase PCR, the RNA is used as a template to make a cDNA copy (through reverse transcriptase)A researcher used polymerase chain reaction (PCR) to test four individual nematodes for the presence of a mutation in Gene Y. When amplified, the fragments of DNA containing the mutated gene alleles were 250 base pairs long and the normal (wild type) alleles were 350 base pairs long. To identify the genotypes of the individual nematodes, the researcher used gel electrophoresis to visualize the PCR results. 1.000 bp 500 bp 300 bp 200 bp 100 bp Remember that these nematodes are diploid organisms and as such, inherited two copies of Gene Y. Which of the following statements is a correct analysis of these results a) Nematode As homozygous for this gene and has two copies of the mutated allele. O b) Nematode C is heterozygous for this gene and has one copy of the mutated allele and one copy of the normal (wild type) allele. O) Nematode D is heterozygous for this gene and has one copy of the mutated allele and one copy of the normal (wild type) allele. ) d) Nematode B is homozygous for this…A fellow lab worker brings you DNA containing what might be a similar gene in Leopard Geckos (XG). She asks you to see if you can amplify it using the same primers you used in frogs and the Bearded Dragon. You run the PCR and then analyze the product by running the DNA on an agarose gel. As a control, you run out the PCR product you amplified in Bearded Dragons and a DNA “ladder” of DNA pieces of known sizes. The gel results are shown below. Marker = DNA ladder; Lane A = Bearded Dragon PCR product; Lane B = Leopard Gecko PCR results. In the box below, determine the sizes of the PCR products shown in Lanes A & B. 16c. Yes or No - based on the results shown in the gel above – would you say the frog PCR primers for gene X amplified the same gene in the Bearded Dragon?
- At the end of an experiment, you extract DNA from ten yeast colonies. You divide the DNA from each colony into two tubes, and send all twenty samples for sequencing. How many experimental replicates do you have?You are studying a genetic disease and trying to determine its location on a chromosome using restriction mapping. You digests DNA from patients with restriction enzymes, runs it on a gel, blots, and probes with a specific oligonucleotide. Which grandparents are the original carriers? (ie responsible for the disease) Grandparents 1,8 3,5 2,6 4,7 Parents 1,5 2,7 Children 2,5 2,5 1,2 5,7 1,2 1,7 5,7 8. 6. 4 2| O mom's mom (maternal grandmother) and dad's dad (paternal grandfather) O mom's dad (maternal grandfather) and dad's dad (paternal grandfather) O mom's mom (maternal grandmother) and dad's mom (paternal grandmother) O mom's dad (maternal grandfather) and mom's mom (maternal grandmother)After you design the PCR primers, you run the PCR on fluid from the patient’s nasal swab. Next, you need to evaluate the results. PCR makes billions of copies of just one sequence in a sample. Since you know the sequence, you also know the length (number of nucleotides) of the region you copied. There are about 130 nucleotides in the N gene fragment. This is typically stated as 130 base pairs. How can you visualize DNA and estimate its size? Load the DNA sample into an agarose gel (similar in consistency to jello) and apply an electric current to the gel. The DNA is charged and will move through the gel. The longer the DNA fragment, the more slowly it moves. The DNA is visualized by adding a fluorescent dye to your sample that sticks to DNA. When you look at the gel under UV light, the DNA should glow. What is the charge of a DNA molecule? Based on #1, would you expect DNA to be drawn to the (+) or (-) electrode of the gel electrophoresis chamber?
- Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser. b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments. c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR. d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser. e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.If you were offered the chance to have the genome of your newborn sequenced at a cost of 1,000, would you do so?You are performing PCR for the first time using some new primers that are 25 nucleotides in length with an estimated melting temperature of 65 0C to amplify a 200 nucleotide gene. After PCR, you run an agarose gel on the samples and observe a faint band at 25 nucleotides. What is the best possible explanation for the results? The primers hybridized to multiple sites on the DNA template. Too much template DNA was added to the PCR mixture. The primers dimerized preventing DNA transcription from occurring. A nuclease was in the solution causing degradation of the DNA.
- we done an experiment where we extracted the DNA from strawberry, grape, and peas. With the strawberries, we were able to find its DNA in the solution very easily because it was floating on the top. But for the grapes we were only able to see very small clumps which we weren't able to collect. And for the peas, we weren't able to see anything or collect any of their DNA. all I need is for help with the CER(claim, evidence, and reasoning) thank you!!what does it mean if our Pcr product was 6.1 nanograms per micrometers and our pcr script was 4.9 nanogramse per micrometers. we are doing a cloning experiment with Ldh dnaDo all of them True/False 31) The process by which an electrical charge is used to introduce DNA into a cell to produce a transgenic organism is called electroporation.Answer: 32) Reproductive cloning is used to produce large amounts of mammalian proteins from transgenic agricultural animals such as cattle.Answer: 33) In gene addition, homologous recombination is used to remove the original gene and replace it with the cloned gene.Answer: 34) All stem cells have the potential to differentiateAnswer: 35) A bone marrow transplant involves the transfer of multipotent stem cellsAnswer: 36) The fact that in mammalian systems multiple genes may compensate for the loss of a gene is called gene redundancy.Answer:
