IMPORTANT POINTS TO REMEMBER: •Homozygous (one band)- the size of the band (lower or higher in the gel) determines if normal (both alleles cut by MSt II, hence band lower) or diseased (both alleles uncut and hence at the higher position in gel) •Heterozygous (two bands in the same lane) denotes a carrier (lower band from normal allele which is cut by RE & upper sickle cell allele band is uncut)
Southern Blotting & Detection of sickle cell disease: Please interrupt the following results and draw a Punnett square, and answer the questions.
•Discuss figures (gel figure and Punnett square) one by one.
•You must provide discussion for each lane of the gel, how you make out normal, carrier, disease (allelic patterns- why so?), control
IMPORTANT POINTS TO REMEMBER:
•Homozygous (one band)- the size of the band (lower or higher in the gel) determines if normal (both alleles cut by MSt II, hence band lower) or diseased (both alleles uncut and hence at the higher position in gel)
•Heterozygous (two bands in the same lane) denotes a carrier (lower band from normal allele which is cut by RE & upper sickle cell allele band is uncut)
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