In a monolayer of cultured epithelial cells, the cells may or may not have formed tight junctions. What experimental test could be used to determine it the cells have functional tight junctionstructures?Place a small dye (<1 k Da in molecular weight) into the culture media of cells cultured on a glass coverslip and use live imaging to monitor whether the dye can penetrate into theO cytoplasm of the cells, as Would be expected if the cells have functional tight junctions.Place a small dye (<1 k Da in molecular weight) on the apical side of a cell monolayer cultured on a permeable membrane and use live imaging to determine if the dye remained on theO apical side, as would be expected if the cells have functional tight junctions.Place a large dye (>1 k Da in molecular weight) into the culture media of cells cultured on a glass coverslip and use live imaging to monitor whether the dye can penetrate into theO cytoplasm of the cells, as would be expected if the cells have functional tight junctions.Inject a cell with a small dye (<1 kDa in molecular weight) and use live cell imaging to track whether the dye moves into neighboring cells, as would be expected if the cells have functionalO tight junctions.

One of the types of cell junctions is the tight junction. Tight junctions are otherwise called…

In a monolayer of cultured epithelial cells, the cells may or may not have formed tight junctions. What experimental test could be used to determine if the cells have functional tight junction structures? Place a small dye (<1 k Da in molecular weight) into the culture media of cells cultured on a glass coverslip and use live imaging to monitor whether the dye can penetrate into the O cytoplasm of the cells, as would be expected if the cells have functional tight junctions. Place a smalldye (<1 k Da in molecular weight) on the apical side of a cell monolayer cultured on a permeable membrane and use live imaging to detemine if the dye remained on the O apical side, as would be expected if the cells have functional tight junctions. Place a large dye (>1 k Da in molecular weight) into the culture media of cells cultured on a glass coverslip and use live imaging to monitor whether the dye can penetrate into the O cytoplasm of the cells, as would be expected if the cells have functional tight junctions. Inject a cell with a small dye (<1 kDa in molecular weight) and use live cell imaging to track whether the dye moves into neighboning cells, as would be expected if the cells have functional O tight junctions.

In a monolayer of cultured epithelial cells, the cells may or may not have formed tight junctions. What experimental test could be used to determine if the cells have functional tight junction structures? Place a small dye (<1 k Da in molecular weight) into the culture media of cells cultured on a glass coverslip and use live imaging to monitor whether the dye can penetrate into the O cytoplasm of the cells, as would be expected if the cells have functional tight junctions. Place a smalldye (<1 k Da in molecular weight) on the apical side of a cell monolayer cultured on a permeable membrane and use live imaging to detemine if the dye remained on the O apical side, as would be expected if the cells have functional tight junctions. Place a large dye (>1 k Da in molecular weight) into the culture media of cells cultured on a glass coverslip and use live imaging to monitor whether the dye can penetrate into the O cytoplasm of the cells, as would be expected if the cells have functional tight junctions. Inject a cell with a small dye (<1 kDa in molecular weight) and use live cell imaging to track whether the dye moves into neighboning cells, as would be expected if the cells have functional O tight junctions.

Human Physiology: From Cells to Systems (MindTap Course List)

9th Edition

ISBN:9781285866932

Author:Lauralee Sherwood

Publisher:Lauralee Sherwood

Chapter3: The Plasma Membrane And Membrane Potential

Section: Chapter Questions

Problem 7RE

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