In a TLC experiment using a 70:30 mixture of Petroleum ether and ethyl acetate, a student noted the development of spots in the origin also, what can you suggest about this observation
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In a TLC experiment using a 70:30 mixture of Petroleum ether and ethyl acetate, a student noted the development of spots in the origin also, what can you suggest about this observation?
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- Consider the fluorescence spectra for bovine serum albumin (BSA) below. One solution contains phosphate buffered saline (PBS), while the other contains 1M HCL. Why does the fluorescence change the way it does? I.e., why do we observe a higher peak and a shift to the left under the presence of acid?For various species or portions of organisms, different stains have different affinities. Explain this assertion using the example of hematoxylin and eosin staining.Consider the photos here which demonstrate antibiotic sensitivities of Staphylococcus aureus strains as determined by the Kirby-Bauer method. Abbreviations are as follows: C = chloramphenicol; CC = clindamycin; CZ = cefazolin; E = erythromycin; NOR = norfloxacin; OX = oxacillin; P = penicillin; RA = rifampin; SAM = sulbactam-ampicillin; SXT = sulfatrimethoprim; TE = tetracycline; VA = vancomycin. Imagine that only two cellular changes occurred in the original strain (the first image, on the top) that resulted in the resistance pattern of the strain in the second image (on the bottom). Which combination of mechanisms could explainthese results?Choose one or more: A.expression of efflux pumps B.overproduction of PABA C.production of β-lactamase D.altered penicillin-binding protein E.modification of either 50S or 30S ribosomal subunits F.altered DNA gyrase
- Which among the general tests for alkaloids can be used as a visualizing agent in detecting alkaloids at TLC chromatogram? Explain.Explain the effects of ampicillin and X-gal in the following 4 different LB media plates LB LB + X-gal LB + Amp LB + Amp + X-galYou have an order for 1 gram of Cefazolin in D5W 100 ml. You have added 5 ml of sterile water to the 1 gram vial to reconstitute powder. However the recommended manufacturer’s diluent amount is 10 ml of sterile water for a final concentration of 100 mg/ml. How would reconstituting the vial with 5 mls affect the concentration and the final calculated dose?
- For a TLC experiment, what is the purpose of the pentane extraction of spinach juice? Why wouldn’t we want to spot an aqueous solution onto the silica gel?You are asked to design an assay to measure the concentration of WonderDrug in various different pharmaceutical preparations using either its UV-visible absorption or its fluorescence emission. Explain when a UV-visible absorption based assay would be the more appropriate and when a fluorescence based assay would be more appropriate.You perform a Bradford assay to determine the concentration of isolated α-lactalbumin. You use 50 μL of a two-fold diluted solution of α-lactalbumin in the assay. You generate a standard curve with the following equation for the line: y = 0.163x + 0.082. The absorbance of your sample was 0.674 AU. What is the concentration of α-lactalbumin, in mg/mL, in your sample? Give your answer to three significant figures.
- While performing the experiment " Detection of ammonium sulphate in Milk", a pink color result is observed. What can we conclude from this observation?propose a hypothesis regarding the organism (escherichia, staphylococcus, pseudomonas, bacillus) that will demonstrate greater resistance to UV and why? if the hypothesis is supported, what specific experimental results will be observed? and what serves as the control treatment?1- Explain the germicical action of agno3 and hgcl2 2- Why does concentrated hno3 acid stain the kin yellow 3- explain the use of heat as an agent of sterlization. cite examples where heat is used as an agent of sterlization 4- offer an explination why picric acid and tannic cid are used in the treatment of burns . 5- cite some practical applicayions of detnaturation