In microscopy, what could be the possible reason why we cannot completely resolve the specimen under the Oil Immersion Objective (OIO) when, in fact, it was taught that the OIO has a resolving poewer? 2. When using the OIO, we can only focus on one group or parts at a time. (that is, the other parts are blurred) Whys is it so
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1. In microscopy, what could be the possible reason why we cannot completely resolve the specimen under the Oil Immersion Objective (OIO) when, in fact, it was taught that the OIO has a resolving poewer?
2. When using the OIO, we can only focus on one group or parts at a time. (that is, the other parts are blurred) Whys is it so?
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- 1. How will the following affect resolution during microscopy?i. Closing or opening the diaphragmii. Raising or lowering the condenserii. Increasing or reducing the light intensity 2. During the calibration of an ocular micrometer, two (2) stage micrometer divisions were observed to line up with ten (10). If the graduation interval on the stage micrometer is 0.01mm, calculate: i. The calibrated value of the ocular micrometer in um.ii. The size of Pseudomonas aeruginosa which has occupied 5 ocular divisions. 3. What are the methods of staining?3. Please number these steps, in the order they should be completed when using a microscope. Remove specimen, lower stage, wrap cord, and return covered microscope to numbered shelf. Move to other objective lenses to increase magnification. Rotate lowest-magnification objective lens into position (probably 4x or 10x). Clean all lenses with lens paper. Place specimen on stage. Adjust chair to a comfortable height. Use coarse focus knob to adjust stage until an image is visible. Be careful to use only the fine focus knob with the longest objective lenses.3. Using the information provided, calculate the size of the objects viewed. Hint you will need to calculate the FOV. These cells are being viewed under high power. Use the length of just one cell to estimate the number of cells that can fit into the FOV. Scanning power objective = 5X; Low power objective = 40X; High power objective = 100X; Eyepiece = 10X; Low power field of view (FOV) = 1.5 mm
- 1.Why is wavelenth the main limiting factor on limit of resoltuin in light microscopy? 2.Assuming that all other variables remain constant, explain why light of shorter wavelengths will produce a clearer image that light of longer wavelenths. 3. Why aren't the magnification of both ocular lenses of a binocular microscope used to calculate total magnification?2. Complete Table 1 below by supplying the characteristics of each objective. Table 1. Numerical characteristics of microscope objectives. Focal length (mm) Working distance (mm) Linear magnification (X) Numerical aperture (N.A.) Features (color of the band, diameter of the opening of the lens, etc.) LPO HPO ΟΙΟ3. Using the information provided, calculate the size of the objects viewed. Hint you will need to calculate the FOV. A.The cell is being viewed under high power. Scanning power objective=4x; Low power objective= 10x; High power objective= 40x; Eyepiece= 5x; Low power field of view (FOV)= 4.2 mm
- 1. How is the letter “e” on the slide oriented when you see it with the naked eye as you mount it on the stage (i.e., is it right side up or upside down)? 2. How is the letter “e” on the slide oriented when you see it under low or high power magnification? 3. What effect, if any, does the compound light microscope have on the orientation of the image?1. What does moving the coarse focus and fine focus do? Why is it important to remember the difference between coarse and fine focus? 2. What is the resolution of a microscope? How is resolution affected byadjusting the light, coarse and fine focus? please answer on ur own words, do not copy from google or i will downvotea. Do you open both eyes or close one eye when viewing a specimen under a microscope? Why? Why not? b. In using a compound microscope, do you start with the low power objective (LPO) or the high power objective (HPO)? Why or Why not? c. When using 60X, do you it together with the coarse adjustment knob or fine adjustment knob? d. Why must you slowly turn the fine adjustment knob? e. Is natural light or is artificial light used when using the microscope?
- For what purpose would you adjust each of the following microscopecomponents during a microscopy exercise?a. Iris diaphragm:b. Coarse-adjustment knob:c. Fine-adjustment knob:d. Condenser:e. Mechanical stage control:B. Write the function/s of each part of the microscope listed below. a. Eyepiece b. Draw tube c. Hemispheric prism housing d. Dust shield e. Revolving Nose Piece f. Objectives: Scanner LPO HPO ΟΙΟ g. Arm h. Coarse Adjustment knob i. Fine adjustment knob j. Slide holder & clip k. Rear knob I. Front knob m. Central aperture n. Condenser o. Iris diaphragm p. Mechanical Stage q. Mirror r. Mirror rack s. Stage adjustment knob t. Base3. Using the information provided, calculate the size of the objects viewed. Hint you will need to calculate the FOV. b. These cells are being viewed under high power. Use the length of just one cell to estimate the number of cells that can fit into the FOV. Scanning power objective = 5X; Low power objective = 40X; High power objective = 100X; Eyepiece = 10X; Low power field of view (FOV) = 1.5 mm