In the preparation of a bacterial smear, why is there a need to fix the bacteria to the slide? Aside from passing the slide over a flame, what are the other ways of fixing the bacteria to the slide?

Q: What is the purpose of the alcohol (OH) in the gram staining process?

A: Usually, microbes are colorless, so it’s very difficult to see them even after magnifying under…

Q: What is the usual stain used for staining a sputum smear?

A: Essentially, sputum comprises of a combination of saliva and mucus. additionally named as phlegm, It…

Q: layer

A: The crystal violet eventually escapes from cytoplasm of the cell, if left too long for…

Q: Why do you have to boil the agar solution before dispensing it into tubes?

A: Agar is the medium which can used to grow the micro organisms in petri plates. The selected…

Q: What is the correct shape of the unknown bacterium growing on your MacConkey agar?

A: Macconkey Agar This agar includes bile salts, which are found in the gut. Bile salts help in…

Q: in the clinic, the acid-fast stain is used to diagnose infection with which bacterium?

A: The Ziehl-Neelson (ZN) stain is the most commonly utilized of the different types of AF staining…

Q: What are the disadvantages of doing a total microbial count by examining wet mounts under the…

A: Ans- Wet mounts have the following drawbacks: The benefit of being able to observe movement can also…

Q: In Gram staining technique, Decolorizing agent step should take Select one: O a. 60 seconds O b. 30…

A: Usually, Acetone is used as a decolorizer but some prefer ethanol and acetone in a 1:1 ratio.…

Q: In gram staining, could colors other than red be used as a counterstain?

A: A counterstain is a stain with color contrasting to the principle stain, making the stained…

Q: Noah wanted to transfer Staphylococcus aureus from a broth to an agar plate. He picked up the broth…

A: The aseptic techniques are applied in the laboratory setup to reduce the risk of contamination. It…

Q: Describe the bacteria observed in different staining procedures. Please refer to the photo -…

A: Bacteria can be stained by a variety of stains so that they can be visualised in contrast to the the…

Q: Why was a blood agar, rather than a nutrient agar, plate used for the culture from your mouth?

A: Cultured bacteria are any bacteria that grow in the lab by the microbiologist using the required or…

Q: When bacteria from a throat swab are streaked on blood agar, why is the agar stabbed several times…

A: The initial identification of the streptococcal and enterococcal species is based on their hemolytic…

Q: shape

A: There are different shapes of bacteria and different classes of bacteria on its shape These shapes…

Q: If you were working with an unlabeled simple stained smear, would you be able to identify the…

A: Staining is used to colour biological specimen such as cell to identify them. Simple staining…

Q: Imagine that you are Gram staining a mixed culture of both gram-positive and gram-negative bacteria.…

A: The bacteria cell envelope is a complex multilayered structure and based on this structure the…

Q: What is the purpose of the quadrant streak plate method? - How could this method be used in a…

A: In quadrant streak plate method, first of all the plate on which bacteria has to be grown that is on…

Q: Name all the reagents used in Gram-staining and their roles in the staining procedure.

A: Staining is a strategy used to improve contrast in examples, for the most part at the minuscule…

Q: In gram staining, if bacteria retain the primary stain after decolorizing agent treatment. Then the…

A: Answer: GRAM STAINING = It is the differential staining method to differentiate between gram…

Q: Supposed you are to observe an unknown bacterial slide. In your initial observation, it appears to…

A: Microorganisms are defined as organisms that cannot be viewed by unaided eyes and require a…

Q: What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and…

A: What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and…

Q: What is the correct shape of the unknown bacterium growing on your mannitol salt agar

A: Mannitol salt agar also known as MSA is commonly used selective agar in microbiology. This material…

Q: Why did we both stab and streak our blood agar plates when inoculating them with bacteria?

A: Blood agar is a nutrient medium which contain essential nutrients for bacterial growth and blood of…

Q: Would you use an inoculating loop to transfer bacteria to an agar deep tube?

A: Agar deep tube is a glass tube which is filled with solid medium. The medium is solid due to…

Q: Why does fixation of the positive dye help bacteria stick to the slides?

A: Dyes in microbiology are for staining cells; they are used based on the chemical properties and the…

Q: How is air contamination prevented when an inoculating loop is used to introduce or take a bacterial…

A: Biotechnology is a branch of biology, including the use of living organisms to produce products.…

Q: How would you expect the staining properties of 24-hour culture of Bacillus subtilis or the other…

A: Bacillus subtilis is a very well bacteria that is widely used as a Gram-positive bacteria model…

Q: The Gram staining procedure involves multiple steps with use different reagents. Complete the table…

A: Step 1 Christian Gram in 1884 developed a technique of staining bacteria called Gram staining.…

Q: Bacterial cells can form colonies on the following media EXCEPT: Select one: Nutrient broth Agar…

A: The nutrient broth is the liquid nutrient media that lacks the solidifying agent. It is used for…

Q: What is the main advantage of using the slide culture technique for identifying molds?

A: Side Culture - It is a rapid method of preparing fungal colonies for examination and identification.…

Q: Why are smears of capsulated bacteria not heat-fixed before staining as opposed to Gram staining?

A: Gram staining is a differential staining technique developed by Danish bateriologist Hans Christian…

Q: What would be the expected outcome of an endospore stain test If the bacteria is a pathogenic…

A: Endospore stain is a differential stain that is used to image endospores in bacteria. A few genera…

Q: How do Blood agar & EMB agar help us to identify organisms more specifically than a gram stain?

A: How do Blood agar & EMB agar help us to identify organisms more specifically than a gram stain?

Q: you are given a mixed culture of s. aureus, E. coli and P. aeruginosa. how would you isolate each…

A: In the study of microorganisms, a mixed culture is one that contains more than one type of organism…

Q: After performing a Gram stain on a Gram negative bacteria, the specimen does not show pink or purple…

A: steps that are included in the Gram staining are- applying a primary stain (crystal violet) to a…

Q: What is the function of a mordant, and which reagent serves this purpose in the Gram stain…

A: An extensively used microbiological method of staining is called a Gram stain. It significantly…

Q: How do we reconcile the number of colonies in the plates to the actual number of bacteria in the…

A: Microbes may enter the body in a variety of ways and cause infection everywhere, but antigen and…

Q: Since the dyes for the Gram stain are not specific to the bacteria theystain, what mistakes in the…

A: Gram staining is a technique used for staining the microbial samples. It was performed by the…

Q: the chemicals used in the gram staining method?

A: The bacteriologist Hans Christian Gram was developed gram staining method in 1884. the Gram staining…

Q: What is Gram staining?Describe step by step the procedure.

A: GRAM STAINING METHOD:- This is a differential staining that was developed by Dr. Hans Christian Gram…

Q: why is gram staining important

A: Gram staining is a laboratory technique which is used to differentiate between different types of…

Q: What advantage does the oil immersion objective have over the low power objective when doing…

A: Introduction:- Bacteria are prokaryotic and very small sized organism's . They can not be visualised…

Q: If zn stain is positive from a sputum sample, what would be your pre-treatment of choice to isolate…

A: Zn stain test also called as Ziehl-Neelsen test is a rapid method to detect the acid fast bacteria.

Q: Why is low microbial population in milk samples a limitation for microscopic count?

A: The bacteria present in milk are lactococcus , lactobacillus, streptococcus, leuconostoc and…

Q: What color wouod gram negative cells appear if the decolorizing step was left out of staining…

A: Gram staining method: This technique is useful in identifying two groups of bacteria based on the…

Q: why is it important that you disinfect your work area both before and after working with bacteria?

A: Bacteria are present everywhere in each and every corner,present on non-living surfaces and as well…

Q: What staining technique is used here? What is the primary stain, decolorizing agent, and counter…

A: Gram Staining is the common, important, and most used differential staining techniques in…

Q: What is this media? What type of media is it? Which type of organism can grow on this plate?…

A: Media is... Agar This is the solids type of Media. Organism type... Streak plate technique is used…

Q: Why did you perform the catalase test on colonies growing on nutrient agar plates but not on the…

A: Catalase test is used to identify the presence of catalase enzyme in the bacteria. Catalase is an…

Microbial Growth

The development of microbial growth is influenced by various natural conditions (both physical and synthetic) like pH, temperature, saltiness, gas focus (O2, CO2, and H2), nutrients, water action, and assimilation. Knowing the distinctive physical and chemical factors that influence the development and endurance of microbial growth in the climate will assist with reinforcing endeavors outfitted towards their control. These lines lessen or diminish their harmful impacts on humankind, creatures, and the climate.