You are a technician in a biochemistry lab running receptor binding experiments. The target membrane-bound receptor has beenpartially purified from mouse, rat, and human cell lines. Using the same radioactive ligand in a saturation binding assay for eachspecies' receptor, you generate the binding data in the table. The dependent variable, Y, is the fraction of binding sites occupiedby the ligand.LigandY for mouseY for ratY for humanconcentrationreceptorreceptorreceptor(nM)0.200.0480.290.170.500.110.500.331.00.200.670.504.00.500.890.80100.710.950.91200.830.970.95500.930.990.98Determine the Ka for the human receptor in this binding experiment.human receptor KdnM

Kd is equilibrium dissociation constant which is measured to determine the tendency of a molecule to…

Answered: Ligand Y for mouse Y for rat Y for…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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