Lysozyme purified by ion-exchange chromatography was collected from 380 mL egg white. i) The initial rate of the reaction catalyzed by 0.15 mL of 50-fold diluted egg white was 0.320 OD unit min-¹. ii) The initial rate of the reaction catalyzed by 0.15 mL of 20-fold diluted pH 10.5 filtrate was 0.900 OD unit min-¹. If there were 60 mLs of pH 10.5 filtrate collected, what percentage of original activity for egg white was recovered? Show all calculations.

Lysozyme purified by ion-exchange chromatography was collected from 380 mL egg white. i) The initial rate of the reaction catalyzed by 0.15 mL of 50-fold diluted egg white was 0.320 OD unit min-¹. ii) The initial rate of the reaction catalyzed by 0.15 mL of 20-fold diluted pH 10.5 filtrate was 0.900 OD unit min-¹. If there were 60 mLs of pH 10.5 filtrate collected, what percentage of original activity for egg white was recovered? Show all calculations.

Appl Of Ms Excel In Analytical Chemistry

2nd Edition

ISBN:9781285686691

Author:Crouch

Publisher:Crouch

Chapter12: Spectrochemical Methods

Section: Chapter Questions

Problem 7P

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