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- Differentiate between the limit of resolution of the typical light microscope and that of the unaided human eyes the relationship between the depth of field and magnification is that When magnification increases, the depth of field decreases. Name two procedures (steps) you should do to achieve the maximum resolution when using the oil immersion lens State the relationship between the working distance of an objective lens and its magnification power “The coarse focus knob can be used to adjust the focus when using any of the objective lenses”. Is this statement true or false? If it is false, please correct it. When using the oil immersion lens but you cannot focus, what should you should not do? And what you should do? What does this statement mean: “Your microscope is parfocal”? What is the significance of condenser lens? Multiple choice question: The most useful adjustment for increasing image contrast in low power magnification is _______ Closing down the diaphragm Closing one…Explain when to use bright-field, phase-contrast, dark-field, fluorescence, transmission electron, and scanning electron microscopy for a given situation. What is an example of this situation?Which of the following is true about the relationship between depth of field and magnification? There is no relationship. When magnification increases, depth of field decreases. When magnification increases, depth of field increases. Stereomicroscopes offer a lesser depth of field than compound microscopes. The depth of field of a microscope determines how much magnification it can achieve.
- What is the importance of using an immersion oil in 100x magnification? Describe the changes in the field of view and the amount of available light when going from low to high power using the compound microscope. Give some limitations in the use of microscope. Conclusion about microscopePrevious question: For example, you can estimate the field of view diameter at 400x total magnification using the measured diameter at 100x total magnification. The magnification is 4 times greater so the field of view will be 4 times smaller. What is the field of view under the 40X objective of the microscope. - The Answer I got for this question was 3/4mm.If the ocular lens of a microscope has 10X magnifying power, and the 40X objective lens is being used for observation, what is the total magnification for the viewed image? 10x 43x 1000x 400x
- PRELAB: Calculating Magnification Each lens makes the object look larger by a specified amount. To determine the total magnification, multiply the magnification of the eyepiece and the objective being used. Table 1: Microscope Magnification Magnifying power Objective Objective Eyepiece Total name Scanner 4 X Low power 100 X High power 40 χ PRELAB: Estimating the Size of an Object using a Microscope You will not be able to use a ruler to directly measure the size of a cell. Instead, you will use an indirect method by comparing the cell to the size of something you already know. The diameter of the microscope's field of view as seen through the eyepiece is a convenient length to use. A very small unit of measurement, the micron (µm) is used for measuring microscopic objects. The conversions are: 1 um = 0.001 mm 1 mm = 1000 µm Table 2: Field of View Diameters Objective Diameter name millimeter (mm) micron (um) Scanner 4 Low power High power 0.5 To estimate the size of an object, divide…Total magnification of a microscope is determined by Multiple Choice dividing the objective magnification value by the ocular value. multiplying all objective magnification values together. adding all objective magnification values together. none of the above.Calculate the diameter of the field of view for each total magnification on your microscope in millimeters (mm) and then convert this value to micrometers (um): 4,500 um Scanning (40X): 1.8 mm x 100X/40X = 5 mm = %3D Low power (100X): FOV diameter = 1.8 mm = 1,800 um 1.8 mm x 100X/400X = 0.45 mm =450 um 180 um High power (400X): 1.8 mm x 100X/1000x = 28 mm = Oil immersion (1000X): %3D Draw and estimate the length of a single Euglena (high power) and Paramecium (low power): Paramecium Euglena total magnification total magnification FOV diam. um FOV diam. um length um length um
- Calculate the diameter of the field of view for each total magnification on your microscope in millimeters (mm) and then convert this value to micrometers (um): 4.5 mm = 4,500 um Scanning (40X): 1.8 mm x 100X/40X = Low power (100X): FOV diameter 1.8 mm = 1,800 um High power (400X): 1.8 mm x 100X/400X = 0.45 mm = 450 um Oil immersion (1000X): O.18m 180 um 1.8 mm x 100x/1000X = Draw and estimate the length of a single Euglena (high power) and Paramecium (low power): Paramecium Euglena total magnification total magnification 400x yoox FOV diam. FOV diam. um uni length length umAlternatives used on a light microscope might improve vision by using a different light source. Name two types of light sources.Explain the differences between Brightfield, Darkfield, and Phase-Contrast Microscopy.
