min+ = confers resistance to minocycline cep+ = confers resistance to cephalexin van+ = confers resistance to vancomycin The following table shows the results of your experiments.  Transformation of the recipient cells was conducted, using the DNA of the donor cells.  You next selected for recipient cells that had undergone transformation by plating the transformed cells on media containing minocycline. This enabled you to select for min+ cells. The min+ cells were then replica plated onto media containing cephalexin or vancomycin in order to determine the antibiotic resistance phenotypes with respect to these other two antibiotics Questions a) Of the three potential orders of these genes, which orders are possible and which are impossible based on your knowledge of the relative distance? b) Finally, determine the correct order of the three genes. Please include a

Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter16: Adult And Pediatric Dosages Based On Body Surface Area
Section: Chapter Questions
Problem 7.4P
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min+ = confers resistance to minocycline

cep+ = confers resistance to cephalexin

van+ = confers resistance to vancomycin

The following table shows the results of your experiments.  Transformation of the recipient cells was conducted, using the DNA of the donor cells.  You next selected for recipient cells that had undergone transformation by plating the transformed cells on media containing minocycline. This enabled you to select for min+ cells. The mincells were then replica plated onto media containing cephalexin or vancomycin in order to determine the antibiotic resistance phenotypes with respect to these other two antibiotics

Questions

a) Of the three potential orders of these genes, which orders are possible and which are impossible based on your knowledge of the relative distance?

b) Finally, determine the correct order of the three genes. Please include a DIAGRAM to illustrate how you deduced the correct gene order, as well as an explanation of your reasoning.

Antibiotic Resistance Present in These Transformants
Minocycline only
Cephalexin and Minocycline
Minocycline and Vancomycin
Cephalexin, Minocycline, and Vancomycin
Genotype of transformants
min+ cep-van-
min+ cep+ van-
min+ cep-van+
min+ cep+ van+
Number of Colonies
545
680
19
76
TOTAL: 1320
Transcribed Image Text:Antibiotic Resistance Present in These Transformants Minocycline only Cephalexin and Minocycline Minocycline and Vancomycin Cephalexin, Minocycline, and Vancomycin Genotype of transformants min+ cep-van- min+ cep+ van- min+ cep-van+ min+ cep+ van+ Number of Colonies 545 680 19 76 TOTAL: 1320
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