Objective lens X has a limit of resolution equal to 0.2 mm while objective lens Y has 30 µm. a. Which among the two can magnify a mosquito wing in finer detail? b. Which among the two would show a greater area of the mosquito wing?
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- Objective lens A has a limit of resolution equal to 0.2 mm while objective lens B has 30 μm. a. Which among the two can magnify a mosquito wing in finer detail? b. Which among the two would show a greater area of the mosquito wing?What is the diameter of the field of view (DFV) of a 1000x objective lens if the DFV of a 400x objective lens is 500 μ? Express your answer in mm.The field of view (FOV) is the entire circular image we see when looking into the eyepiece. The diameter of the FOV gets smaller as we increase magnification. It can be measured by using a stage micrometer like a ruler, measuring from edge to edge. Notice that the stage micrometer is 1000 microns (µm) in length, and the field of view under the lowest magnification is 5000 µm. Describe how we measure it?
- Consider a bi convex lens with radius of curvatures |R1| = 30 mm and |R2| = 180 mm, ct = 5 mm and glass is N-BK7. This is used as camera lens in order to image a man who is at 1 km away from the camera. We want to resolve 10 cm on his body. Assume that λ = 550 nm. Using zemax, determine the MTF value for the resolution of R = 80 lp / mm in image plane which is placed at paraxial focus. (Hint: First, find the diameter of the lens) Which one: a.32.1 % b.28.7 % c.12.3 % d.58.5 % e.46.8 % f.57.2 % g.20.9 % h.15.6 %What is the rule for determining total magnification? A. Ocular lens magnification + objective lens magnification = total magnification B. Ocular lens magnification - objective lens magnification = total magnification C. Ocular lens magnification x objective lens magnification = total magnificationDefine each of the following terms: A) What is resolution and how is resolution related to the wavelength of light used to illuminate the sample? B) What is the magnification of the specimen if you are using a 40x objective and a 10x eyepiece? C) How is the numerical aperture (NA) of a lens related to its ability to gather light from a specimen?
- In spectrophotometry, what is the role of the green background in the set-up? What does it represent in the actual spectrophotometer?Use the iris diaphragm to attain the best illumination of the letter “e” on the scanning (4x) objective. Open and close the iris diaphragm and notice the changes in contrast of the letter “e”. How did the appearance of the letter “e” change under the various levels of illumination? 1 Repeat the above procedure on the high-power (40x) objective. When increasing magnification, what happens to the light available? Why?Explain how total magnification is calculated. Select an answer. a. It is the magnification of the objective lens multiplied by the magnification of the condenser lens b. It is the magnification of the objective lens multiplied by the magnification of the ocular lens c. It is the magnification of the objective lens used d. It is the magnification of the ocular lens used
- a: What are the components of a Romanowsky stain? b: What is the optimum pH for staining with Giemsa. c: Name two conditions which will affect the quality of your staining. d: What is a panoptic stain. Give one example. e: Name two blood parasites that can be demonstrated using the Giemsa stain.How can a UV-Vis spectrophotometer be applicable in determining the absorbance/concentration of a colorless sample? Please answer in detail and clearly. Thank you so much!It was found that the units on the ocular micrometer and the units on the stage micrometer matched up 40 units to 1.0 mm at 100 power. (So 40 ocular units equals 1.0 mm.) If the wing of a dead fruit fly was measured to be 2.5 ocular micrometer units at a magnification of 100, what is the length of the wing in mm?
