% T Analyst Date 101.9 101.0 100.5 101.5 -100.0 99.0 98.5 99.5- 98.1+ 4000 3500 3000 University of Rochester Thursday, October 14, 2021 nyinyentual 2500 2000 1658 cm-1 University Of Rochester 1058 Sample 1058 By University
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- 6. C8H100 11 TRANSMITTANCE LOD HSP-04-492 0+ 10 4000 9 3000 8 5 7 6 : 5 ppm 2000 4 HAVENUMBERI-l 2: 2 1 3 1500 2 سهر 1 1000 0 500 200 T 180 CDS-06-431 DEPT-90 T 160 DEPT-135 140 120 100 لك -2 peaks u ppm 80 60 40 20 0Molecular Formula: C10H14 8 LOD TRANSMITTANCE1% D 4000 STRUCTURE: mult. t 2.72 q 6.90 S d t ppm 1.18 7.11 7.53 3000 H 6 4 1 2 1 5 2000 SPECTRUM 5 4 PPM HAVENUMBERI-L 1500 3 1000 500OA B. OC D. CE. Compound G is an Aldehyde Compound H is a Carboxylic acid Compound is an alcohol A Compound B is a ketone Compound is an Adehyde A Compound B is a Ketone B Compound B is an Aldehyde Compound is a Ketone Compound is an Alcohol Compound B is a Carboxylic acid
- Match the following terms to the correct statement Molar absorptivity Absorbance Transmittance 1. Fraction of light that is transmitted by a substance 2. logarithmically related to PT/Po The constant relating absorbance and the product of the concentration and the pathlength when the concentration is in moles/I and the pathlength is in cm 3.3500 3000 2500 Wavenumber cm-1 2000 1500 1000 500 3920.67 3082.32 2336.05 2235.94 1699.33 1655.52 1631.23 1407.26 1255.15 1189.54 1103.98 998.71 811.98 471.79 60 Transmittance [%] 80 70 90 fundamny BRUKER 100Potassium dichromate and potassium permanganate have overlapping absorptionspectra in 1M H2SO4 . K2Cr2O7 has an absorption maxima at 440 nm, and KMnO 4 has a band at 545nm (the maximum is actually at 525 nm, but the longer wavelength is generally used whereinterference from K2C2O7 is less). A mixture is analyzed by measuring the absorbance at these twowavelengths with the following results: A 440 =0.405, A 545 =0.712 in a 1-cm cell. The absorbances ofpure solutions of K2Cr2O7 (1x10 -3 M) and KMnO 4 (2.00x10 -4 M) in 1 M H 2 SO 4 , using the same cell gavethe following results: A Cr,440 = 0.374, A Cr,545 = 0.009, A Mn,440 =0.019, A Mn,545 = 0.475. Calculate theconcentrations of dichromate and permanganate in the sample solution.
- 4000 3500 3000 2500 Wavenumbers (cm-1) 2000 20 1500 1000 500 1705.32 1354.36 1135.21 1476.83 997.91 2969.16 %Transmittance 1274.53 832.71 1427.75 953.40 553.28 790.43 1218.83 1585.98 894.05 671.73 780.29 463.73 100 CSpectrum 28 100% 75%- 50%- 25% 0% 3500 2500 1750 1250 750 4000 3000 2000 1500 1000 50 Wavenumber (cm) NH2 Transmittance (%)A standard 3 mL cuvette is two-thirds filled with a 1 in 5 dilution of 70 mM oxyhaemoglobin. The absorbance is measured at 580nm. A few drops of a live yeast suspension is added to the cuvette (without significantly changing the volume). The absorbance is remeasured at the same wavelength as above and is found to be 0.40, a decrease of 0.2 on the first measurement. What is the molar absorbance coefficient of the oxyhaemoglobin at 580 nm? (do not enter the units in the answer, 2 decimal places)
- Tablet powder containing 100mg of promazine hydrochloride is ground to a paste with 10 ml of 2 M HCl. The paste is then diluted with 300 ml of water, shaken for 15 min and finally made up to 500 ml. A portion of the extract is filtered. Then, 5 ml of the filtrate is taken and diluted to 200 ml with 0.1 M HCl. – Absorbance reading=0.855 is read at a wavelength wavelength of 251 nm – a (1%, 1 cm) value of promazine.HCl at 251 nm=950 – Stated content of promazine.HCl per tablet=60 mg – Weight of 24 tablets=1.500g – Weight of tablet powder taken for assay=180 mg expected content in tablet taken is: 172.8 mg 1000mg 100 mg 100g Concentration in diluted tablet extract is: 0.1g/mL 0.9 g/100ml 1.11 g/mL 0.9 mg/100ml Concentration in original tablet extract is: 36g/mL 36 mg/100ml 19.38 mg/100ml 16.15 mg/mL Amount of promazine.HCl in original extract is: 172.8mg 180mg/100ml 96.90mg 180mg percentage of stated content 99.1 % 96% 80.75mg/100ml 104.2%Alsorbance Absorbance Absorption Spectrum of CoCl,(aq) Beer's Law Plot of CoCl, at = 560 nm 045 05 04 03 y = 48571x+0j0038 02 200 300 400 T00 S00 005 01 Wavelength inm) Concentration (M) Figure 1: Absorption Profile for cobalt (II) chloride Figure 2: Cobalt (II) chloride standard curve (Beer's Law Plot)* www.ucirvine.instructre.com 2. Place an arrow at the maximum absorbance and extrapolate the wavelength max. Amax = (be sure to include the unit). 3. Determine by concentration of an unknown solution of cobalt (II) chloride having an absorbance of 0.25. Method 1: Extrapolation (mark the concentration on the plot) b. Method 2: Calculation (show your work) a. Life University CHM 111 March 2021JM 4: C₂H₁2O 100 % Transmittance 95 90 85 80 75 70 65 60 Janne DRAW ONE POSSIBLE STRUCTURE ATR 4000 3750 Sigma-A ALL RIGHTS RESERVED 3500 any 1000 1250 1500 1750 2000 2250 2500 Wavenumbers (cm-1) © 2023, Sigma-Aldrich Co. ALL RIGHTS RESERVED 2750 3000 3250 750 500