Sanger sequencing (aka the chain termination) a. is a method for for determining the sequence of nucleotides in a piece of DNA of intrest. b. requires use of special ddNTPs (dideoxy nucleotides) that have special florecent lables (dyes) attached to them and also lack a 3' hydroxyl. c. requires fragments to be run through a tube containing a gel matrix where their fluorescent dyes are illuminated with a laser, which can be used to produce a chromatogram. d. all of the above are true e. none of the above are true

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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Sanger sequencing (aka the chain termination)

a. is a method for for determining the sequence of nucleotides in a piece of DNA of intrest.

b. requires use of special ddNTPs (dideoxy nucleotides) that have special florecent lables (dyes) attached to them and also lack a 3' hydroxyl.

c. requires fragments to be run through a tube containing a gel matrix where their fluorescent dyes are illuminated with a laser, which can be used to produce a chromatogram.

d. all of the above are true

e. none of the above are true

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