Select all of the statements that are true about a buffer solution. A buffer solution resists small changes in pH. A buffer solution always changes color when the pH changes. A buffer solution reacts with basic solutions. A buffer solution has a pH of 7. A buffer solution reacts with acidic solutions.
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- When dissolved in water, a(n) _____ donates H+ and a(n) ______ accepts H+. a. acid; base b. base; acid c. buffer; solute d. base; bufferThree proteins with an overall charges of 0, -5, and -8 are passed through an anion exchange column equilibrated in a buffer with low ionic strength and eluted with an increasing gradient of salt in a buffer. Which protein will elute last from the column? 1. Protein with no charge. 2. Protein with eight negative charges. 3. Protein with five negative charges. 4. Both charged proteins will elute at the same time. O 2 O 4 O 3 O 1You had a second solution with an unknown concentration of Protein X that had to be diluted 4x before you could measure the absorbance. The diluted solution had an absorbance of 0.76. What is the concentration of the diluted solution in ug/ml and what is the concentration of the original solution
- You need to prepare 500 ml PBS buffer, a solution commonly used in biological research. The buffer helps to maintain the pH, and the osmolarity, as well as ion concentrations of PBS buffer, match those of the human body. PBS buffer consists of the following components: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4. How would you prepare the buffer?Choose the FALSE statement An indicator can be used to monitor a buffer range When pH equals PKa of weak acid, the buffer capacity is the greatest Polyprotic acids have multiple stoichiometric points in their titration curve At the mid-point of titration of weak acid by strong base, the pH of the solution -does not equal the pKa of the weak acid The moles of titrant equals moles of titrateA 0.10 M NaCl solution is (hypertonic/hypotonic/isotonic) to a 0.1M glucose solution.
- You have just added substance X to a glass of water. The pH of the water changed from 7 to 4. you conclude that substance X is a because it caused the amount of H+ ions in the water to for each choice, the item in front of the semicolon (;) goes into the first space. the item behind the semicolon goes into the second space. for example if a choice says- "buffer; increase", buffer goes into 1st space and increase goes into 2nd space buffer; neither increase or decrease base; decrease base; increase acid; decrease O acid; increaseTable 2. Volume of BSA, protein content, and absorbance readings of reference solutions Solution Volume of BSA standard solution (μL) Protein content(μg/mL) Absorbance value At 595 nm 1 0 0 0 2 10 1 0.022 3 30 3 0.065 4 50 5 0.106 5 70 7 0.178 6 100 10 0.299 7 120 12 0.380 Make a graph by plotting the absorbance values versus the BSA protein content (in μg) for theseven reference solutions. When constructing the graph, be…Which of the following statements about pH is (are) true: pH is a measure of free hydrogen ion concentration none of these are true both of these are true An increase in hydrogen ion concentration means a decrease in pH scale units