Solubility Test Ninhydrin test iodoform test Observations + or - no colored spots on filter paper clear pale-yellow solution clear red-orange solution Br2/CH2C12 (dark) Molisch test turbid colorless solution Tollen's Test clear, colorless solution
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- Analysis of the pathological constituents GIVE THE FOLLOWING: - Name of Test/Test Reagent - Positive Result 1) Protein (Note: Presence of protein in urine is termed as proteinuria or albuminuria) A. Heat and Acetic acid test Fill a test about ¾ full of urine. Heat the upper portion gently to boiling for 1 -2 minutes being careful not to shake the tube. Rotate the tube to prevent overheating. A turbidity may be due to albumin, phosphates or carbonates. Add 3 drops of acetic acid drop by drop while boiling between each drop. If turbidity disappears, it is due to carbonates and phosphates. B. Heller’s nitric acid test Place 1 ml of urine sample in a test tube. Hold the test tube in an inclined position and carefully pour 1 drops of conc HNO3 down the side of the test tube. Note formation of white ring at the zone of contact of the two solutions which is an indication of the presence of protein.Which of the following is FALSE about the Nitrate Reductaion Test, procedure and interpretation? Question 2 options: A Nitrate medium contains 0.1% potassium nitrate as a substrate B Reagent A and B are used to determine the presence of nitrite C When zinc is added, it will react with any nitrate and turn red D A tube with red after zinc is a positive test for nitrate reduction E A tube that turns red after reagent A and B is a positive testWhat is the color result of 1% soultion of casein,albumin ang gelatin in biuret test? Results and interpretation with reference
- PART III. PRECIPITATION BY ALCOHOL AND OTHER ORGANIC SOLVENTS TUBE NO. 1. 1 ml each of TREATMENT RESULT and OBSERVATION 5 mL of 95% ethanol 5% solution of egg albumin 2. 1 ml each of 5 mL of 95% 5% solution of acetone egg albumin Generalization:A. Experimental Results Sample No. 1 2 3 4 5 1 Sample No. Sample Identity 2 3 + Table 1. Results of the Qualitative Tests of Unknown Lipid Samples Copper acetate Test Greenish-blue color Colorless Colorless Greenish-blue color Colorless 5 Acrolein Test Hubl's Test Ammonium Molybdate Test No precipitates No precipitates No precipitates No precipitates Yellow precipitates No distinct odor Pungent odor No distinct odor No distinct odor Pungent odor Brown solution Light pink color Brown solution Light pink color Light pink color Table 2. Sample Identity of Unknown Lipid Samples Rationale Liebermann- Burchard Test No color change No color change Bluish-green color No color change No color changeChoose the best combination of depyrogenation methods: 0.45 um filtration + 0.22 µm filtration OUV+0.45 um filtration 0.22 µm filtration + UV treatment 0.45 um filtration + distillation 0.22 µm filtration + distillation
- Chemical tests See bottom of page 8 for instructions on how to complete this table. C. Biuret test Solution turins olive greeni Yeu ight Pur ple A. Iodine test B. Benedict's test Description of Solution turne Slohtion turns positive result Tube No. and (copy from Contents dark Purple or brovniornd asbiutrldeins Pp. 7-8) decp blue-blakk or tomoto of Short +/- Appearance blue (from p. 9) らish | +/- | Appearance blae Appearance +/- 1 - Orense 2 - Glucose Starch orunde Orange 3 - ders Brtelt blue blue blve bue 4 - alanine oransC- blue - 5. blue iout bue t orange Why was it important to have tube number 1 in each test? (Don't just say "because it's the control", and don't copy from the lab instructions-write out your own understanding of why and how that tube was used.)What is the main purpose of Huble's Test? How does it differ/similar to Unsaturation Test? How do this tests is conducted? What is the positive results for this test? What is the principle behind this test ?QUALITATIVE TESTS: Biuret Test Ninnhydrin TestXanthoproteic TestMillon's TestHopkin's-Cole TestSakaguchi TestNitroprusside TestFohl's TestTest for AmidePauly's Test
- What does the phrase "pipetting up and down" mean and why is this technique used? The phrase "pipetting up & down" means to triturate. Th technique is used to mix (or homogenize) a solutic 7 On what part of a microcentrifuge tube should you write a label? Describe the order in which you filled the tubes in Step 2 of Procedure 4. Did this order result in maximum efficiency? If not, what order would be most efficient? 9 What does the phrase "spinning down" mean and why is this technique used? The word 'spinning down" means, To diminish in energy i to slow down o out; to be gradually ended or concelled. It is used to reduce its spin speed from that required for reading and writing. 10 Why was the comb placed in the middle rather than at one end of the gel for this electrophoresis experime- The comb is in the center of the gel Since the dyes used have positive or negative charges and can therefore migrate in differenImmediately after stirring 1 hour after stiring Original misture Suction filiration Figure 1 Figure 2 Figure 3 Various tests are performed on the mixtures as shown in the figures above. Identify any correct explanations of these tests below. [Check all statements that are true.] In figure 1, a laser is passed through two tubes of clear liquid. The liquid in the tube on the left is a solution, while the liquid in the right tube is not a solution. O In figure 2, a recently stirred mixture is allowed to stand for one hour. The original mixture is a solution while the mixture after 1 hour is not a solution. In figure 3. a mixture is filtered using suction filtration. A solid remains on the filter. The original mixture was a solution.Procedure:1. Prepare 5 test tubes. Place 1 ml of 1% starch and add 10 drops of saliva toeach tube. Mix thoroughly.2. Place the first tube in ice water, the 2ndtube leave at room temperature, the 3rdtube in 40°C , the 4thtube at 60°Cwater bath and the 5thtube boil for 2minutes..3. Leave the 4 tubes in their respective temperatures for 30 minutes. The 4thtubeallows to stand for 30 minutes after heating for 2 minutes.4. Test the contents of each tube with iodine and benedict’s tests.