Take a look at this graph. The reactant curve is plotted in red, while the product curve is plotted in green. We need to determine the best wavelength to measure the product at. The product should exhibit good absorbance at the chosen wavelength. The investigated substance should be the only substance absorbing well at the chosen wavelength. 0.900 0.00 0500 0.400 0.300 0.200 0.100 0.000 $10 Wavelength is n 450 B00 Absorbance (AU)

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
icon
Related questions
Question

his is the result of an experiment in a spectrophotometer. please explain the graph (red and green one)

Awesome work! You're an analytical genius! The product of the reaction displayed an
absorbance maximum at 595 nm, while the starting reactant had relatively little
absorbance in this part of the spectrum. This made 595 nm the perfect wavelength for
our investigation.
Let's head back to the lab to get on with our verification experiments.
Transcribed Image Text:Awesome work! You're an analytical genius! The product of the reaction displayed an absorbance maximum at 595 nm, while the starting reactant had relatively little absorbance in this part of the spectrum. This made 595 nm the perfect wavelength for our investigation. Let's head back to the lab to get on with our verification experiments.
Take a look at this graph. The reactant curve is plotted in red, while the product curve is
plotted in green.
We need to determine the best wavelength to measure the product at. The product
should exhibit good absorbance at the chosen wavelength. The investigated substance
should be the only substance absorbing well at the chosen wavelength.
465 nm
0.900
0.800
0.700
595
0.600
0.400
0.300
0.200
0.100
0.000
Wavelergth is nn
Absorbance (AU
Transcribed Image Text:Take a look at this graph. The reactant curve is plotted in red, while the product curve is plotted in green. We need to determine the best wavelength to measure the product at. The product should exhibit good absorbance at the chosen wavelength. The investigated substance should be the only substance absorbing well at the chosen wavelength. 465 nm 0.900 0.800 0.700 595 0.600 0.400 0.300 0.200 0.100 0.000 Wavelergth is nn Absorbance (AU
Expert Solution
steps

Step by step

Solved in 2 steps

Blurred answer
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON