The data given below was generated by a student in Bio 240L during a Bradford Assay. A standard curve was generated by preparing a set of standard samples of protein (bovine serum albumin) and adding the dye Coomassie Brilliant Blue, When the protein binds to Coomassie Brilliant Blue it absorbed light at 595 nm with an extinction coefficient of 43,000 M-1 cm 1. After the dye binding reaction was complete, absorbance values for each protein standard solution were measured at 595 nm and recorded. Standard # 1 2 3 4 Concentration of protein binded to Coomassie Brilliant Blue (M) 6.35x10-7 1.23x10-6 3.85x10-6 5.68x10-6 Absorbance at 595 nm 0.093 0.176 0.560 0.813 Prepare a standard curve with the student generated data. What is the slope from the standard curve rounded to four sig figs and no units? 1.4 143398 6.972x10-6 1.434x105

The data given below was generated by a student in Bio 240L during a Bradford Assay. A standard curve was generated by preparing a set of standard samples of protein (bovine serum albumin) and adding the dye Coomassie Brilliant Blue, When the protein binds to Coomassie Brilliant Blue it absorbed light at 595 nm with an extinction coefficient of 43,000 M-1 cm 1. After the dye binding reaction was complete, absorbance values for each protein standard solution were measured at 595 nm and recorded. Standard # 1 2 3 4 Concentration of protein binded to Coomassie Brilliant Blue (M) 6.35x10-7 1.23x10-6 3.85x10-6 5.68x10-6 Absorbance at 595 nm 0.093 0.176 0.560 0.813 Prepare a standard curve with the student generated data. What is the slope from the standard curve rounded to four sig figs and no units? 1.4 143398 6.972x10-6 1.434x105

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter26: Molecular Absorption Spectrometry

Section: Chapter Questions

Problem 26.32QAP

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