The following data is for the oxidation of catechol (the substrate) to o-quinone by the enzyme o-diphenyl oxidase. Additional data is also provided for when the reaction is run in the presence of p-hydroxybenzoic acid (PBHA). All data was acquired by monitoring the change in absorbance at 540 nm. Die volgende data is vir die oksidasie van katekol (die substraat) na o-kinoon deur die ensiem o- difenyloksidase. Bykomende data word ook verskaf vir wanneer die reaksie uitgevoer word in die teenwoordigheid van p-hidroksibensoësuur (PBHA). Alle data is verkry deur die verandering in absorpsie by 540 nm te monitor. [catechol] (mM) Rate (AAU/min) Rate (AAU/min) Uninhibited PBHA 1,01 65,02 38,27 0,49 47,11 24,60 0,32 38,44 16,99 0,25 32,84 13,88

The following data is for the oxidation of catechol (the substrate) to o-quinone by the enzyme o-diphenyl oxidase. Additional data is also provided for when the reaction is run in the presence of p-hydroxybenzoic acid (PBHA). All data was acquired by monitoring the change in absorbance at 540 nm. Die volgende data is vir die oksidasie van katekol (die substraat) na o-kinoon deur die ensiem o- difenyloksidase. Bykomende data word ook verskaf vir wanneer die reaksie uitgevoer word in die teenwoordigheid van p-hidroksibensoësuur (PBHA). Alle data is verkry deur die verandering in absorpsie by 540 nm te monitor. [catechol] (mM) Rate (AAU/min) Rate (AAU/min) Uninhibited PBHA 1,01 65,02 38,27 0,49 47,11 24,60 0,32 38,44 16,99 0,25 32,84 13,88

Principles of Modern Chemistry

8th Edition

ISBN:9781305079113

Author:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler

Publisher:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler

Chapter20: Molecular Spectroscopy And Photochemistry

Section: Chapter Questions

Problem 4P

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