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The following diagram shows the two strands of a single DNA molecule, with a pair of PCR primers hybridized at positions flanking the target region. The arrowheads point in the direction of DNA replication, 5’ to 3’. In the figure and table below, the solid line represents DNA from one strand (call it the "top" strand); the dashed line represents DNA of the complementary strand (the "bottom" strand). The number of PCR products double after each cycle of PCR (Ifn=number of cycles and P= number of product molecules, then P= 2"). The double strand DNA (dsDNA) PCR products fall into a number types; after a number of PCR cycles the target-region dsDNA type becomes the most common product. However, in very early cycles other products can be found represented in appreciable numbers. 5´- 5' Double stranded DNA PCR product # after 2 # after 3 # after 4 # after 15 PCR cycles PCR cycles PCR cycles PCR cycles All types of PCR products FIn the table above, enter the number of duplex DNA products of the illustrated type that will be present among the PCR products after a specified number of cycles. For the images in the table, the positions of the ends of the DNA strands are aligned, relative to each other and to the initial figure above. The dotted lines dropping down from the figure shows the alignments. Assume the PCR reaction was started with a single molecule of double stranded template DNA.

The following diagram shows the two strands of a single DNA molecule, with a pair of PCR primers hybridized at positions flanking the target region. The arrowheads point in the direction of DNA replication, 5’ to 3’. In the figure and table below, the solid line represents DNA from one strand (call it the "top" strand); the dashed line represents DNA of the complementary strand (the "bottom" strand). The number of PCR products double after each cycle of PCR (Ifn=number of cycles and P= number of product molecules, then P= 2"). The double strand DNA (dsDNA) PCR products fall into a number types; after a number of PCR cycles the target-region dsDNA type becomes the most common product. However, in very early cycles other products can be found represented in appreciable numbers. 5´- 5' Double stranded DNA PCR product # after 2 # after 3 # after 4 # after 15 PCR cycles PCR cycles PCR cycles PCR cycles All types of PCR products FIn the table above, enter the number of duplex DNA products of the illustrated type that will be present among the PCR products after a specified number of cycles. For the images in the table, the positions of the ends of the DNA strands are aligned, relative to each other and to the initial figure above. The dotted lines dropping down from the figure shows the alignments. Assume the PCR reaction was started with a single molecule of double stranded template DNA.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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The following diagram shows the two strands of a single DNA molecule, with a pair of PCR primers hybridized at positions flanking the target region. The arrowheads point in the direction of DNA replication, 5' to 3'. In the figure and table below, the solid line represents DNA from one strand (call it the "top" strand); the dashed line represents DNA of the complementary strand (the "bottom" strand). The number of PCR products double after each cycle of PCR (If n= number of cycles and P= number of product molecules, then P= 2"). The double strand DNA (dsDNA) PCR products fall into a number types; after a number of PCR cycles the target-region dsDNA type becomes the most common product. However, in very early cycles other products can be found represented in appreciable numbers. 5'- 5' Double stranded DNA PCR product # after 2 # after 3 # after 4 # after 15 PCR cycles PCR cycles PCR cycles PCR cycles All types of PCR products In the table above, enter the number of duplex DNA products of the illustrated type that will be present among the PCR products after a specified number of cycles. For the images in the table, the positions of the ends of the DNA strands are aligned, relative to each other and to the initial figure above. The dotted lines dropping down from the figure shows the alignments. Assume the PCR reaction was started with a single molecule of double stranded template DNA.
Transcribed Image Text:The following diagram shows the two strands of a single DNA molecule, with a pair of PCR primers hybridized at positions flanking the target region. The arrowheads point in the direction of DNA replication, 5' to 3'. In the figure and table below, the solid line represents DNA from one strand (call it the "top" strand); the dashed line represents DNA of the complementary strand (the "bottom" strand). The number of PCR products double after each cycle of PCR (If n= number of cycles and P= number of product molecules, then P= 2"). The double strand DNA (dsDNA) PCR products fall into a number types; after a number of PCR cycles the target-region dsDNA type becomes the most common product. However, in very early cycles other products can be found represented in appreciable numbers. 5'- 5' Double stranded DNA PCR product # after 2 # after 3 # after 4 # after 15 PCR cycles PCR cycles PCR cycles PCR cycles All types of PCR products In the table above, enter the number of duplex DNA products of the illustrated type that will be present among the PCR products after a specified number of cycles. For the images in the table, the positions of the ends of the DNA strands are aligned, relative to each other and to the initial figure above. The dotted lines dropping down from the figure shows the alignments. Assume the PCR reaction was started with a single molecule of double stranded template DNA.
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