The following DNA sequence is from a bacteriophage that infects a pathogenic bacterium and scientists want to know if this bacteriophage could prove to be a potential treatment against it. But first scientists need to discover if different strains of this pathogen have restriction endonucleases that it may use for its own protection. They try 3 different RE’s: a)    EcoR1     b) HaeIII    c) BamH1 Look up the recognition sequences for the 3 Res. Enzymes above and check whether the phage genome (a snippet of which is shown below) will or will not be ‘cut’. Tell me how their experiment worked out and what their conclusion was. G A A A A G G C C A C A A G G C C G T C G A C T T T T A A A A G G C C A C A T G C G G C  T T  T T  C C G G T G T T  C C G G C AG C T  GA A A AT T  T T  C C G G T G T A C G CC

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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The following DNA sequence is from a bacteriophage that infects a pathogenic bacterium and scientists want to know if this bacteriophage could prove to be a potential treatment against it. But first scientists need to discover if different strains of this pathogen have restriction endonucleases that it may use for its own protection. They try 3 different RE’s:
a)    EcoR1     b) HaeIII    c) BamH1

Look up the recognition sequences for the 3 Res. Enzymes above and check whether the phage genome (a snippet of which is shown below) will or will not be ‘cut’. Tell me how their experiment worked out and what their conclusion was.
G A A A A G G C C A C A A G G C C G T C G A C T T T T A A A A G G C C A C A T G C G G C  T T  T T  C C G G T G T T  C C G G C AG C T  GA A A AT T  T T  C C G G T G T A C G CC

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