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A:
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A: *NOTE: Kindly repost for other question Dear Student as per the guidelines we are supposed to answer…
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Q: DNA polymerases used in PCR
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The production in a pcr reaction is:
double strand circular dna
double stranf linear dna
single steand amyisense rna
Step by step
Solved in 2 steps
- PCR primers Below is a 300 base pair fragment of DNA. The top strand is written in the 5' to 3' direction. The bottom strand is written 3' to 5'. There are also two primer sequences; both primers are written 5' to 3'. Note that we are displaying a double-stranded DNA fragment, but primers will only bind to one of the two displayed strands. 5' ACCGȚAGCTATATGCTATCGTGACGTATCGGCGCATTAAȚCGGGATCGAT 3 50 3' TGGCÁTCGATATACOATAGCACTOCATAGCCGCGTAATTÀGCCCTAGCTÀ 5' 5' AGCTÇGCTAGCAGGAGAGAȚATCGÇTCATAGCTCCGATCGATGCCGCTAA 3 3' TCGAGCG ATCGTCCTCTCTÁTAGCGAGTATCGAGÓCTAGCTACGGCGATİ 5' 100 5' TATAGCTCTÇTGCGGATATÇGCATATACCẠ AGGCCCTACGTATGTAGCTA 3 150 3' ATATČGAGAGACOCCTATAGCGTATATGGTTCCGGGATGČATACATCGAŤ 5' 5 TGCGTATATÇGGAGAGTCCTGGATATGGAGCTTGACTGCAGAGAGCTCGA 3 200 3' ACGCÁTATAGCCTCICAGGÁCCTATACCTCGAACTGACGTCTCTCGAGCT 5' 5' TATGCGCTTAGGCCGTATATGCTTGGGGAAAGCTCTATGTATGCTATGTG 3 3. ATACGCGAATCCGGCATATACGAACCCCTÍTCGAGATACATACGATACAC 5' 250 5' TGCATGTGCTATGCAACGTTCOGATTGCGȚAGCAGTAATAGCGCCGATTG 3 300 3'…PCR primers Below is a 300 base pair fragment of DNA. The top strand is written in the 5' to 3' direction. The bottom strand is written 3' to 5'. There are also two primer sequences; both primers are written 5' to 3'. Note that we are displaying a double-stranded DNA fragment, but primers will only bind to one of the two displayed strands. 5' ACCOȚAGCTATATOCTATCOTGACOTATCOGCOCATTAAȚCGGGATCGAT 3 3' TGGCATCGATATACGATAGCACTGCATAGCCGCGTAATTAGCCCTAGCTẢ 5 50 5' AGCTCGCTAGCAGGAGAGATATCGCTCATAGCTCCGATCGATGCCGCTAA 3 100 3' TCGAGCGATCGTCCICTCTATAGCGAGTAICGAGGCTAGCTACGGCGATİ 5' 5' TATAGCTCTCTGCGGATATÇGCATẠTACCAAGGCCCTACGTATGTAGCTA 3 150 3' ATATČGAGAGACGCCTATAGCGTATATGGÍTCCGGGATGČATACATCGAŤ 5 5' TGCGȚATATÇGGAGAGTCCTGGATAT GGAGCTTGACTGCAGAGAGCTCGA 3 200 3' ACGCATATAGCCTCICAGGACCTATACCTCGAACÍGACGICTCTCGAGCİ 5' 5' TATGCGCTTAGGCCGTATATGCTTGGGGAAAGCTCTATGTATGCTATGTG 3 250 3' ATACGCGAATCCGGCATATACGAACCCCTITCGAĞATACATACG ẢTACAČ 5' 5' TGCATOTGCTATOCAACGTTC GGATTGCGȚAGCAGTAATAGCGCCGATTO 3' 300 3'…Pcr primers are: single strand 15-25 bases long incorporated into the newly synthesized DNA strand none above all above
- During a PCR reaction, the step in the cycle where dNTPs are added to the primer 3’ ends to fill in the strand complementary to the template by DNA polymerase is called: Annealing Extension Hybridization Melting.Which of the following ARE part of a typical PCR reaction mixture? DNA ligase dNTPs (mix of nucleoside tri-phosphates) RNA primers made by primase enzyme template DNA, often from cells collected from hair, cheek swab, or blood 2 DNA primers polymerase enzymeIn a conventional PCR reaction cycle, which of the following step is NOT needed for a successful PCR reaction? anneal amplification refrigeration O denature
- PCR technique is based on DNA transcription. True FalseWhich of the following is NOT a characteristic of PCR primers? Short synthetic oligonucleotide Typically 18-25 bases in length Double stranded DNA Unique homology to the DNA template Sequence with ~50% G:C contentIn a reaction tube, a PCR reaction recreates the DNA replication process inside a cell. Therefore, a PCR reaction needs to have the following essential components: DNA polymerase DNA template Forward and Reverse PCR primers dNTPs, including dATP, dCTP, dTTP, and dGTP MgCl2 True O False
- During the second step of PCR, the temperature is lowered in order to: denature the taq polymerase elongate the primers make a longer polymer of the DNA attach the primers denature the double-stranded DNAsample containing at least one molecule of DNA serves as the starting material for PCR. True FalseWhich of the following is/are true regarding a PCR reaction performed to amplify a DNA plasmid? There may be more than one correct answer; select all that apply. Two primers are needed to facilitate double stranded DNA extension. Denaturation, when the primers bind to the DNA template, is performed at the highest temperature. Extension is the step when the polymerase catalyzes nucleotide incorporation during polymerization. ATP, UTP, GTP, and CTP are all required for polymerization.