Time (min) Degassed tank -1 Air on 0 1 Oxygen uptake rates (OUR) and oxygen transfer rates (OTR) were determined in a fermenter with air bubbled through a bioreactor to supply oxygen for the growth of Candida utilis yeast. In the first set of data below, the fermenter is filled with growth media (aqueous solution) but no cells, and is degassed, removing all oxygen, then at time zero, air enters through the sparge at the bottom of the tank, and the dissolved oxygen (DO) level is measured as a function of time. A second experiment is done with the same fermenter, where the cells are growing in the fermenter at steady state in the stationary phase, and at time zero, the air is cut off, and the DO level is measured for a few minutes before the air is turned on again. The saturation dissolved oxygen concentration (C*) for the conditions used was 4.2 mg/L. Using the measured DO values, determine: A. the maximum oxygen transfer rate, OTR (which occurs when there is no dissolved oxygen) INITIAL STATE Tank filled with growth ← media (no cells) Cells in tank reach steady state concentration Time DO level (mg/L) (min) DO level (mg/L) 0 -1 4.2 0 Air off 0 4.2 0.56 1 4.12 B. the volumetric transfer 2 1.01 2 4.03 coefficient, ka 3 1.43 3 3.91 C. the oxygen uptake rate, 4 1.88 4 3.77 OUR, for the reactor with 5 2.21 5 3.64 cells at steady state 6 2.58 6 3.51 7 2.87 Air on 7 3.35 8 3.14 8 3.46 9 3.35 10 3.51 11 3.65 12 3.78 13 3.9 14 3.995 15 4.07 16 4.13 17 4.17 18 4.2

Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
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Time
(min)
Degassed tank -1
Air on
0
1
Oxygen uptake rates (OUR) and oxygen transfer rates (OTR) were determined in a fermenter with air bubbled through
a bioreactor to supply oxygen for the growth of Candida utilis yeast. In the first set of data below, the fermenter
is filled with growth media (aqueous solution) but no cells, and is degassed, removing all oxygen, then at time
zero, air enters through the sparge at the bottom of the tank, and the dissolved oxygen (DO) level is measured
as a function of time. A second experiment is done with the same fermenter, where the cells are growing in the
fermenter at steady state in the stationary phase, and at time zero, the air is cut off, and the DO level is
measured for a few minutes before the air is turned on again. The saturation dissolved oxygen concentration
(C*) for the conditions
used was 4.2 mg/L. Using
the measured DO values,
determine:
A. the maximum oxygen
transfer rate, OTR (which
occurs when there is no
dissolved oxygen)
INITIAL STATE
Tank filled with growth
media (no cells)
Cells in tank reach steady state
concentration
Time
DO level (mg/L)
(min)
DO level (mg/L)
0
-1
4.2
0
Air off
0
4.2
0.56
1
4.12
B. the volumetric transfer
2
1.01
2
4.03
coefficient, ka
3
1.43
3
3.91
C. the oxygen uptake rate,
4
1.88
OUR, for the reactor with
5
2.21
45
3.77
3.64
cells at steady state
6
2.58
6
3.51
7
2.87
Air on
7
3.35
8
3.14
8
3.46
9
3.35
10
3.51
11
3.65
12
3.78
13
3.9
14
3.995
15
4.07
16
4.13
17
4.17
18
4.2
Transcribed Image Text:Time (min) Degassed tank -1 Air on 0 1 Oxygen uptake rates (OUR) and oxygen transfer rates (OTR) were determined in a fermenter with air bubbled through a bioreactor to supply oxygen for the growth of Candida utilis yeast. In the first set of data below, the fermenter is filled with growth media (aqueous solution) but no cells, and is degassed, removing all oxygen, then at time zero, air enters through the sparge at the bottom of the tank, and the dissolved oxygen (DO) level is measured as a function of time. A second experiment is done with the same fermenter, where the cells are growing in the fermenter at steady state in the stationary phase, and at time zero, the air is cut off, and the DO level is measured for a few minutes before the air is turned on again. The saturation dissolved oxygen concentration (C*) for the conditions used was 4.2 mg/L. Using the measured DO values, determine: A. the maximum oxygen transfer rate, OTR (which occurs when there is no dissolved oxygen) INITIAL STATE Tank filled with growth media (no cells) Cells in tank reach steady state concentration Time DO level (mg/L) (min) DO level (mg/L) 0 -1 4.2 0 Air off 0 4.2 0.56 1 4.12 B. the volumetric transfer 2 1.01 2 4.03 coefficient, ka 3 1.43 3 3.91 C. the oxygen uptake rate, 4 1.88 OUR, for the reactor with 5 2.21 45 3.77 3.64 cells at steady state 6 2.58 6 3.51 7 2.87 Air on 7 3.35 8 3.14 8 3.46 9 3.35 10 3.51 11 3.65 12 3.78 13 3.9 14 3.995 15 4.07 16 4.13 17 4.17 18 4.2
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