Using a compound microscope, if I move my sample from down to up (1), in which direction will the sample move when observed through the eyepieces? OUp Down () Left Right
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Q: w is the letter “e” on the slide oriented when you see it with the naked eye as you mount it on the…
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- Using the microscope Answer the following questions as you work through this exercise: 1. How is the letter “e” on the slide oriented when you see it with the naked eye as you mount it on the stage (i.e., is it right side up or upside down)? 2. How is the letter “e” on the slide oriented when you see it under low or high power magnification? 3. What effect, if any, does the compound light microscope have on the orientation of the image?You have placed a microscope slide in a microscope and want to view it at with the 10X objective lens. List the steps that are necessary to set up and focus the microscope properly using the 10X objective lens.PART C: CALCULATING THE DIAMETER OF THE FIELD OF VIEW (FOV)_ The field of view (FOV) is the circular area you can see when you look through the microscope. The diameter of the field of view is different depending on which objective lens you are using. For example, you are using the medium-power objective lens, then the area you can see is actually smaller than if you were using the low-power objective lens. Knowing The diameter of the field of view can help you estimate actual size of objects / cells seen through the microscope. When the revolving nose piece is turned to the low power objective lens, a dear plastic ruler can be placed on the microscope stage (see figure 1). Then, the coarse adjustment knob can be used to focus on the millimeter marks of the ruler making sure that one of the milimeter marks is at the left edge of the field of view (see figure 2). NOTE: Slage cip I-1000 Objects in the FOV are usually measured in micrometers (um). To convert, a FOV in mm, times it by 1000…
- Previous question: For example, you can estimate the field of view diameter at 400x total magnification using the measured diameter at 100x total magnification. The magnification is 4 times greater so the field of view will be 4 times smaller. What is the field of view under the 40X objective of the microscope. - The Answer I got for this question was 3/4mm.a. Why must you slowly turn the fine adjustment knob? b. Is natural light or is artificial light used when using the microscope?In a compound light microscope, which objective focuses closest to the slide?
- You are in charge of buying new microscopes for your company, and you have been comparing different types of microscopes. One important property for microscopy is the resolution, which measures the ability to distinguish two small objects that are close together. Rank the following list of microscopes from lowest to highest resolution. Microscope List (5 items) (Drag and drop into the appropriate area) Transmission electron microscope (uses high energy electron beam) X-ray microscope (uses x-ray absorption to image sample, range 0.01-10 nm) Confocal fluorescence microscope (uses a blue laser as a light source, 488 nm) Level of Resolution 2 LowestWhen using a microscope, does the depth of focus decrease or increase with increasing magnification?a. Do you open both eyes or close one eye when viewing a specimen under a microscope? Why? Why not? b. In using a compound microscope, do you start with the low power objective (LPO) or the high power objective (HPO)? Why or Why not? c. When using 60X, do you it together with the coarse adjustment knob or fine adjustment knob? d. Why must you slowly turn the fine adjustment knob? e. Is natural light or is artificial light used when using the microscope?
- Which part of the microscope is used to adjust the light? (pick one) i. h. a. d. c.Rowena was tasked to measure the actual magnification of her specimen mounted on a slide without drawing or taking a photomicrograph. Which type of microscope should she use? Why? How does it help her get the actual magnification?What is the purpose of using microscopes? If you need to move the microscope, describe how you should carry it. What three things change as you increase magnification? On the compound microscope, what power must be in place when you first find an image in themicroscope? On the compound microscope, which adjustment knob is used for bringing the specimen intofocus? On the compound microscope, which adjustment knob is used for sharpening the image of thespecimen after it is focused? On the compound microscope, which adjustment knob should you use on the high power? On the compound microscope, how do you change the objective lenses? What should be included when sketching or drawing what is seen through the microscope How do you properly use a microscope?