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- The following strip of chromatography paper has been placed in a polar solvent Which pigments the most polar?differentiate between the paper chromatography with other chromatographyA sample to be used for medical imaging is labeled with 18F,which has a half-life of 110 min. What percentage of the originalactivity in the sample remains after 300 min?
- An amino acid mixture of phenylalanine, glycine and glutamic acid is to be separated by paper chromatography.The solvent is less polar than water. Which of these amino acids will have the highest Rf value and which the lowest? Explain.An amino acid mixture of phenylalanine, glycine and glutamic acid is to be separated by paper chromatography. The solvent is less polar than water. Which of these amino acids will have the highest Rf value and which the lowest? Explain.Three sugars (Sugar A, B and C) were applied to a line as part of the set-up of a Paper Chromatography experiment (method was as described in Practical Manual. After running the chromatography and staining, the following Retention factor (Rf) values were determined. Note that the distance travelled by the solvent was equal across the width of the chromatography paper. Sugar A, Rf = 0.23 Sugar B, Rf = 0.65 Sugar C, Rf = 0.27 What can be drawn from the results of this Paper Chromatography experiment? O Sugars A and C had a stronger interaction with the mobile phase, relative to Sugar B. O Sugar B had the strongest interaction with the stationary phase among Sugars A, B, and C. O Sugar B had the strongest interaction with the mobile phase among Sugars A, B, and C. O Distance travelled by Sugar B must have been shorter than that of Sugars A and C. O Sugar B must be a non-reducing sugar given its high Rf value.
- Since the development of the calcium chloride transformation procedure, other procedures have been developed. One is called electroporation. Using your textbook or other sources, describe this procedure for transformation.briefly explain the principle behind this technique; affinity chromatography using a diagram to illustrate.When conducting a flame test for sodium iodide, is the result positive or negative?
- The Cavity Ring-Down Spectroscopy (CRDS) technique offers greater sensitivity compared to other conventional spectroscopy techniques. Explain the underlying mechanism of this.Arrange the following in order of increasing Rf on thin-layer chromatography: acetic acid, acetaldehyde, 2-octanone, decane, and 1-butanol.The most sensitive and reliable instrument for detectingand characterizing flammable residues is the (gas chromatograph, infrared spectrophotometer).