We have 50 X TAE (Tris-Acetate) stock in our laboratory and the laboratory manager asks you to prepare a 2% gel using 1XTAE that you will prepare from agarose and stock. How do you prepare? (tank tray volume 250 ml).
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We have 50 X TAE (Tris-Acetate) stock in our laboratory and the laboratory manager asks you to prepare a 2% gel using 1XTAE that you will prepare from agarose and stock. How do you prepare? (tank tray volume 250 ml).
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- You want to prepare a 15 mL gel containing 0.8% (weight /volume) agarose. How much agarosedo you need to weigh to prepare the solution? Show all your calculations.Hint: Write down the units for 0.8% knowing that agarose is a powder that is mixed with aliquidOur laboratory has a stock of 50 X TAE (Tris-Acetate) and the laboratory manager asks you to prepare a 2% gel using 1XTAE that you will prepare from agarose and stock. How do you prepare? (tank tray volume 250 ml).You have a sample at 50 ng/ul and you would like to load 400ng of this sample on a lane of an agarose gel. You also have TE buffer as diluent and 6x loading dye. Your total sample should be 12ul. Calculate the amounts of each reagent necessary to prepare this sample for gel loading.
- 1) You have been asked to make up four 1.5% agarose gels at 30 ml each. Ethidium Bromide is to be added at 0.5 ug/ml and you have a bottle at 10ug/ml. Write down step-by-step protocol of how to proceed. 2. How would you make up one liter of 1X TAE buffer using a 25X stock?Rx Clindamycin phosphate 4% w/v Propylene glycol 12.9% w/v Purified water 11% w/v Isopropyl alcohol ad 244 mL (Sp.G. = 0.880) Ft. clindamycin gel Sig.: Place thin layer onto affected areas How many grams of isopropyl alcohol are present in a clindamycin gel that has 76 mL of purified water? Assume negligible volume shrinkage as result of mixing these ingredients. Answer: 415.2 (please show all work) ANSWER IS ALREADY GIVEN PLEASE SHOW WORK IN DETAIL HOW IT COMESIf you have 50 ml of pure stock formalin solution how many ml of diluent will you need? What is the total volume of the reagent used you have prepared? Write down your answers with complete solutions and correct labels.
- A vial of Doxorubicin reads 0•5g per vial. Instructions say to reconstitute each 12mg with 2•5ml of NS. How many ml of NS will be needed to reconstitute the vial of the recommended concentration? please show workingMD ordered morphine SO4 gr 2/5 and the pharmacist has a stock solution of gr 1/8 per milliliter of morphine SO4. How many milliliters of the stock solution is required to fill the Rx?How many mg of agarose do you need to weigh out to make 75 ml 1.3% gel?
- As a part of a clinical study, a pharmacist is asked to prepare a modification of a standard 22 g package of a 2% mupirocin ointment by adding the needed quantity of mupirocin powder to prepare a 3% w/w mupirocin ointment. How many mil- ligrams of mupirocin powder are required?With the G, L, + and – reactions, please tell me whether you would expect there to be a band in the agarose gel or not.How many mL of the 1 M glucose stock solution do you need to prepare 100 mL of a 1 mM glucose solution?