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A:
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What are the importance of accurate wavelength measurement in purified DNA sample?
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- What are the importance of accurate wavelength measurement in purified DNA sample? Explain why contaminants must be avoided in DNA samples.What does the 260 nm light detect when measuring the purity of a DNA sample? Please select the single answer that is most correct.At what stage of the culture should bacterial colonies be harvested for plasmid DNA extraction? How about for genomic DNA extraction? What distinguishes the xanthogenate-based methodology from the traditional phenol/chloroform method for isolating DNA from bacteria? What makes potassium ethyl xanthogenate efficient in isolating DNA from a variety of microorganisms?
- What is the application of Gel Electrophoresis in healthcare? Cite at least 2 specific examples.What is the principle of the gel electrophoresis separation technique?In a protocol for DNA sample preparation for agarose gel electrophoresis, what volume of 4X loading buffer must be added to 21 micro L of DNA to obtain a 1X buffer solution?
- What can be the errors and limitations of Gel Electrophoresis practical? Explain in very much detail and also provide some better suggestions.Please briefly explain what gel electrophoresis is and how it works to separate a mixed sample of macromolecules like DNA.What is the source of agarose, the powder used to make the gel? What is the purpose of placing the comb? What is the purpose of the size standard? What chemical is used to stain the gel? What is used in order to see the DNA bands? What are the sizes of your 3 bands? bp, bp, bp