What is the energy cost of transferring I acetyl-CoA across the mitochondrial membrane? Complete the net equation for the biosynthesis of palmitate in rat liver, starting from mitochondrial acetyl-CoA and cytosolic NADPH, ATP, and Co,. 8 Acetyl-CoAgan) + palmitate + 8 COA+15 ADP+15P+14 NADP +2H 8 ADP+8P, + 8H 8 CoA +7 ADP +7P +14 NADPt 8 COA + 14 NADP +15 ADP+ 15P +7H,0
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- Understanding the Mechanism of Hemolytic Anemia Genetic defects in glycolytic enzymes can have serious consequences for humans For example, defects in the gene for pyruvate kinase can result in a condition known as hemolytic anemia. Consult a reference to learn about hemolytic anemia, and discuss why such genetic defects lead to this condition.21) Compute the moles of FADH2, NADH, GTP generated and NADPH consumed in the dissimilation of one mole each of the octadecanoic acid and A3.5-ocatadienoic acid thioesters shown below to CO2 in the mitochondrial matrix space. Enter the product yields of electron carriers and GTP and the total yield of ATP after processing mitochondrial electron-transport chain in the tables provided below COAS COAS Product Total Converslon Yield ATP Product Total Conversion Yleld ATP FADH2 1.5 FADH2 1.5 2.5 NADH 2.5 NADH NADPH -2.5 NADPH -2.5 GTP GTP Total ATP Total ATP 8.What is the purpose of carnitine acyltransferase II? O formation of a fatty acyl-CoA molecule in the mitochondrial matrix after transport of a long chain acylcarnitine molecule from the cytosol O formation of an acylcarnitine molecule from an acyl-CoA molecule in the cytosol prior to transport across the mitochondrial membrane O transport of an acylcarnitine molecule from the inner membrane space to the mitochondrial matrix O emulsification of long chain fatty acids prior to uptake by intestinal epithelial cells
- In beta oxidation of fatty acids in mitochondria, ubiquinone (via FAD / FADH2) from the respiratory chain acts as an oxidizing agent in one oxidation step and NAD + as an oxidizing agent in the other oxidation step. Discuss the exchange of ATP per oxygen molecule consumed in the breakdown of fatty acids into acetyl-COAI in relation to the exchange of ATP perroxygen molecule consumed if only NADH is the electron source for the respiratory chain.Calculate the number of ATPS generated from the metabolic oxi- dation of the four carbons of acetoacetyl-CoA to CO,. Now consider the homolog derived from oxidation of an odd-numbered carbon chain, namely propionoacetyl-CoA. Calculate the net ATP yield from oxidation of the five carbons of this compound to CO,.10 ATP/Acetyl-CoA * 2 acetyl-CoA = 20 ATP 1 GTP/ Acetyl-CoA * 2 acetyl-CoA = 2 GTP 1,5 ATP/ FADH2 * 1 FADH2= 1,5 ATP 2,5 ATP/ NADH * 3 NADH = 7,5 ATP 7,5 + 1,5 + 20 = 29 ATP och 2 GTP All of this is round off, how to calculate without rounding off? 2-Acetyl-CoA and 15 subunits
- upon digestion of starch maltose, one of its degradation products is further hydrolyzed into its monosaccharide components prior to intestinal absorption and entry into the glycolysis. calculate the number of ATP molecules produced from the digestion and complete oxidation of 1 molecule of maltose considering the glycerol-3-phosphate shuttle. question: 3. what is the total number of mitochondrial NADH prodcued after pyruvate is acted upon by the pyruvate dehydrogenase complexWhat mitochondrial enzyme catalyzes this reaction? "ooc, COA "OoC + H* Co2 COA + "00C NH,+ HN Delta-aminolevulinate synthase Porphobilinogen synthase None of these is correct. Uroporphyrinogen III synthaseUpon considering the complete oxidation of one mole of fatty acyl (CoA) of an arachidic acid (20:0). Answer the following lettersa. total no. of NADH produced from the oxidation of all acetyl CoA in the acetyl cycleb. otal no. of FADH2 produced from the oxidation of all acetyl CoA in the acetyl cyclec. total atp yield
- How much ATP is produced from the complete B-Oxidation of myristic acid (C14H2802)? Activation of Fatty Acid ACCOA x (10 ATP/ACCOA) FADH₂ x (1.5 ATP/FADH₂) = NADH x (2.5 ATP/NADH) : = = -2 ATP ATP ATP ATP TOTAL ATP C18 CH, (CH₂)₁4-CH₂-CH₂-C-S-COA || FAD →FADH₂ CH₂(CH₂)₁4-CH=CH-C-S-CoA Each loop of the pathway represents a repetition of Steps 1-4. H₂O OH CH₂(CH₂)₁4-CH-CH₂-C-S-CoA || - NAD+ →→NADH+ H 0 CH3-(CH₂) 14 C-CH₂-C-S-CoA 0 || C16 CH₂(CH₂)14-C-S-CoA COA-SH Acyl CoA trans-Enoyl COA L-B-Hydroxyacyl CoA B-Ketoacyl COA H₁-C-S- + CH₂-C-S-CoA Acetyl CoA New acyl COA C₁4+ Acetyl CoA C12+ Acetyl CoA CO+ Acetyl CoA Cg+ Acetyl CoA C + Acetyl COA C₁ + Acetyl COA 2 Acetyl COAIf oxidation of acetyl-CoA yields 10 ATPs per mole through the citric acidcycle, how many ATPs will be derived from the complete metabolic oxidation of 1 mole of alanine in a mammal? Would the corresponding energyyield in a fish be higher or lower? Why? How much energy would bederived from the metabolic oxidation of 1 mole of isoleucine to CO2, H2O,and NH3? Of tyrosine?Upon considering the complete oxidation of one mole of fatty acyl (CoA) of an arachidic acid (20:0). Answer the following lettersa. total no. of NADH produced from the oxidation of all acetyl CoA in the acetyl cycleb. total no. of FADH2 produced from the oxidation of all acetyl CoA in the acetyl cyclec. total atp yield