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What is the importance of normalization of data in fluorescence spectroscopy
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- What is the importance of sllid width in fluorescence spectroscopyWhat is the difference between Raman Spectroscopy and SERS ( Surface Enhanced Raman Spectroscopy).Fluorescence intensities depends on the molecular structure and its environment. Discuss the factors that can affect the fluorescence intensity and explain how the factors increase or decrease the intensity.
- ) Draw a schematic diagram of a double beam fluorescence spectrophotometer. Explain the design of a spectrofluorimeter for high throughput measurement. Write down the name of two source lamps used in the fluorescence spectrophotometer? How do you calibrate a fluorescence spectrophotometer?.why steroids do not show luminescence on thin layer chromatographic plates?What is the difference between fluorescence and phosphorescence?