Q: Why should the microscope be calibrated for each objective and prior to each use?
A: Introduction Calibration is the process of establishing up an instrument such that it can produce a…
Q: How do you calculate the total magnification of your microscope?
A: Microscope is an instrument used to see objects and microorganisms which cannot be seen by naked…
Q: What’s the aim of using a microscope for examining? What can the kohler illumination technique tell…
A: A microscope is a special optical instrument that enlarges the size of the object and enables us to…
Q: Why is CSLM able to view different layers in a thick preparationwhile bright-field microscopy…
A: The technical field that uses microscopes to observe objects and their areas that cannot be seen…
Q: When using a microscope, does the depth of focus decrease or increase with increasing magnification?
A: Microscope is an instrument used to observe minute organisms which are invisible to the naked eyes.…
Q: What’s the total magnification for 5x and 44x in a microscope
A: When the size of the image is compared to that of the object that created the image in the first…
Q: Which number on the diaphragm allows the lowest amount of light on a microscope ?
A: The diaphragm number is the numerical aperture of the objective. For example 0.1, 0.25, 0.65, and…
Q: What is the limit of resolution for a bright-field microscope?What defines this limit?
A: As a general bright-field microscope, the limit to magnification is around 1300X and the limit of…
Q: List the optical parts of the microscope. How does it achieve magnification? Resolution?
A:
Q: What are the differences of the 4 objective lenses of microscope? How to get the toal magnification…
A: Microscopy is the science, that deals with the visualisation of the objects using microscope, which…
Q: What is the function of condenser in compound microscope?
A: Introduction; Condenser is an important component of compound microscope, that are located above the…
Q: focusing
A: A light microscope is an apparatus or tool used in a biology laboratory to detect, magnify, and…
Q: For which types of specimens is darkfield microscopy preferred over brightfield microscopy?
A: Microscope is an instrument used to view objects and organisms that are too small to be seen by…
Q: riefly describe the functions of the following parts of the microscope: course objective knob,…
A: Microscopy is the technique of using a microscope and it demands an understanding of its working and…
Q: Which of the following is true about the relationship between depth of field and magnification?…
A: The depth of field refers to resolution in the longitudinal plane. It is measured as the distance…
Q: What are the 2 types of Bright-field microscope. Compare.
A: A bright-field microscope is one of the most common types of microscopes that are used today. The…
Q: Ana was tasked to measure the actual magnification of her specimen mounted on a slide without…
A: A light microscope or optical microscope is used to study a thin section of a rock. The section is…
Q: What is the purpose of confocal microscopy technique ?
A: The microscope instrument is used to view the objects, which are not visible under the naked eye.…
Q: why is microtome important in microscopy?
A: Objects or organisms that cannot be viewed by the unaided eyes are studied under the microscope.…
Q: Demonstrate how to view an object with the microscope using all magnifications
A: Answer: Introduction: Microscope is defined as an optical instrument consist of optical lenses for…
Q: Between wet mount and hanging drop slide preparations, which is more resistant to evaporation? Which…
A: Bacteria are member of a large group of unicellular microorganisms. It has cell walls but lacks…
Q: What is the difference between a simple and a compound microscope?
A: A microscope refers to the laboratory instrument that is used to view tiny objects, which cannot be…
Q: Which microscope achieves the highest magnification and greatest resolution?
A: Out of all the microscopy technologies available, Electron Microscope is the best in terms of…
Q: What advantages do confocal and deconvolution microscopy provide in comparison to conventional…
A: Confocal microscopy uses light from laser, elimination of background information away from the focal…
Q: otal magnification of a microscope is determined by Multiple Choice dividing the objective…
A: The tiny and minute organisms that cannot be observed by unaided eyes are the microorganisms. These…
Q: If you are using a microscope and a specimen takes up 1/4 of the field of vview at 1000X, what is…
A: Magnification = 1000X Specimen takes 1/4 of the field of view.
Q: What are the advantages of using super-resolution microscopyversus standard fluorescence microscopy?
A: Fluorescence microscope is a type of microscope that makes use of phosphorescence and fluorescence…
Q: How do the different parts of microscope serve their specific function? Cite examples
A: Introduction Cell is the basic unit of life which perform all kind of physiological and metabolic…
Q: Describe the process of bringing a specimen into focus using a compound light microscope.
A: A microscope is an instrument that can magnify specimen which is otherwise too small to be seen,…
Q: What part of the microscope allows you to move the stage up and down in small increments to aid in…
A: According to the question, we have to mention the part of the microscope that allows to move the…
Q: What is the advantage of phase-contrast microscopyover bright-field microscopy?
A: Phase-contrast microscopy is an optical microscopy technique that converts phase shifts in light…
Q: Using a compound microscope, if I move my sample from up to down (1), in which direction will the…
A: Answer: COMPOUND MICROSCOPE = It is the microscope used light as an illuminating source and used to…
Q: If accessories for darkfield microscopy were not available, how would you construct a simple star…
A: The microscope is an instrument used to view small objects that cannot be seen with an unaided eye.…
Q: What are the different lens of the microscope?
A: Microscope: It is a laboratory optical instrument that is used to examining object that are too…
Q: Explain the differences between Brightfield, Darkfield, and Phase-Contrast Microscopy.
A: * Here are some differences between bright field microscopy darkfield miccroscopy and phase contrast…
Q: Explain in detail simple and differential staining and also improving and adjusting contrast in…
A: Staining is a chemical method which is used to colour the organism or the cell so that it is viewed…
Q: A microscope that remains in focus regardless of whichobjective lens is rotated into place is…
A: The microscope is an almost widespread image of science, addressing our capacity to investigate the…
Q: Why did we calculate, rather than measure, the diameter of the field of view on medium and high…
A: A microscope is an instrument used to visualize objects that are extremely small in size and cannot…
Q: Consider the following microscope: ocular power = 10X low power objective = 20X high power objective…
A: A microscope is used to magnify and see such objects that cannot be seen by naked eyes. The process…
Q: How is confocal scanning laser microscopy differentfrom fluorescence microscopy? In what ways are…
A: Microscope is an analytical instrument which is used to analyse, study and visualize internal…
Q: What is the formula used to describe the resolution of a microscope lens, and what are the…
A: The term resolution is used in microscopy to characterize a microscope's capacity to identify…
Q: Rowena was tasked to measure the actual magnification of her specimen mounted on a slide without…
A: A microscope is a piece of scientific equipment which magnifies very small objects that are too…
Q: Why is methylene blue used to stain cells? What does it bind to?
A: Answer: Methyl blue is used to stain the cell because it is basic dye which can easily bind with…
Q: What advantage do fluorescent dyes and fluorescence microscopy provide in comparison to the chemical…
A: Microscope is the widely used laboratory instrument in the molecular biology, it is mainly used to…
Q: What is the principle of image formation on transmission election microscope and scanning electron…
A: The general biological microscope helps in the visualisation of the image of a particular sample…
Q: How does differential interference contrastmicroscopy differ from bright-field microscopy?
A: Microscopes are used to observe objects at cellular level such as the nucleus, mitochondria and…
Q: Describe how the images obtained using a confocal microscope would appear different to those…
A: There are many objects that cannot be seen with naked eye. To see such objects an instrument like…
Q: Rowena was tasked to measure the actual magnification of her specimen mounted on a slide without…
A: A simple microscope is a magnifying glass with a short focal length and a double convex lens. The…
Q: What is the purpose of using microscopes?
A: Apologies. We only answer one question at a time. we will answer the question 1 as the exact one…
Q: How do stains used for light microscopy compare withthose used for electron microscopy?
A: The micro-organism is the organism that is too small to be seen with the naked eye. It is viewed by…
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- If the total magnification of a microscope is 700X and the ocular lens magnifies by a factor of 10x, what is the magnification of the objective lens that was used? Paragraph BIUWhat are the advantages of using super-resolution microscopyversus standard fluorescence microscopy?What is the advantage of phase-contrast microscopyover bright-field microscopy?
- If a specimen is being viewed under a 40x objective, and the microscope has a 10x ocular lens, what is the total magnification? 1x 4x 10x 100x 400xHow will the following affect resolution during microscopy? I) Closing or opening the diaphragm II) Raising or lowering the condenser III) Increasing or reducing the light intensityWhat is the highest magnification of the following microscopes?Bright fieldDark fieldPhase-contrastFluorescenceConfocalScanning EMTransmission EMScanned-Probe
- Discussion: 1) What does calibrating the microscope mean? Why do we calibrate the microscope? 2) What is the ocular calibration scale for each objective? Why is important to calibrate each objective?What advantages do confocal and deconvolution microscopy provide in comparison to conventional fluorescence microscopy?3D dimensionality is a limitation of the compound microscope. Depth of field, DOF, describes dimensionality form top to bottom and can be observed with colored cross threads. Observe the crossed thread slide on low power (4x), then on medium power (10x), then on high power (40x objective magnification). Which crossed fiber is on top? How do you know?
- briefly describe the functions of the following parts of the microscope: course objective knob, condenser lens, diaphragm, objective lens, illuminator, fine adjustment knob, oil immersion.How does the compound microscope achieve magnification? resolution? Cite your reference/s if possible.Rowena was tasked to measure the actual magnification of her specimen mounted on a slide without drawing or taking a photomicrograph. Which type of microscope should she use? Why? How does it help her get the actual magnification?