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- Transmittance (%) 100% 75% 50% 25% 0% 4000 3500 3000 OH 2500 2000 Spectrum 3B 1750 حسيمة 1500 Wavenumber (cm-¹) 1250 .OH 1000 OH 750 5000.8 0.6 0.4 0.2 0.0 3000 2000 1000 Wavenumber (cm-1) ОН NH2 `NH2 A В C TransmitancePotassium dichromate and potassium permanganate have overlapping absorptionspectra in 1M H2SO4 . K2Cr2O7 has an absorption maxima at 440 nm, and KMnO 4 has a band at 545nm (the maximum is actually at 525 nm, but the longer wavelength is generally used whereinterference from K2C2O7 is less). A mixture is analyzed by measuring the absorbance at these twowavelengths with the following results: A 440 =0.405, A 545 =0.712 in a 1-cm cell. The absorbances ofpure solutions of K2Cr2O7 (1x10 -3 M) and KMnO 4 (2.00x10 -4 M) in 1 M H 2 SO 4 , using the same cell gavethe following results: A Cr,440 = 0.374, A Cr,545 = 0.009, A Mn,440 =0.019, A Mn,545 = 0.475. Calculate theconcentrations of dichromate and permanganate in the sample solution.
- 3.) The accuracy of a spectrophotometer is evaluated by preparing a solution of 60.06 ppm K2Cr2O7 and measuring its absorbance at a wavelength of 350 nm in a cell with a pathlength of 1.00 cm. The expected absorbance is 0.640. What is the expected molar absorptivity of K2Cr2O7 at this wavelength? 4.) A solution’s transmittance is 35.0%. What is the transmittance if you dilute the solution to half of its initial concentration?100 - 80 60 40 20 10 15 20 25 30 35 40 45 50 55 60 m/z Absorbance / % 50- 100+ 4000 3000 2500 2000 1500 1000 500 Wavenumbers / cm Relative Intensity / %Describe the origin of shot noise in a spectrophotometer. How does the relativeuncertainty vary with concentration if shot noise is the major noise source?
- 6. C8H100 11 TRANSMITTANCE LOD HSP-04-492 0+ 10 4000 9 3000 8 5 7 6 : 5 ppm 2000 4 HAVENUMBERI-l 2: 2 1 3 1500 2 سهر 1 1000 0 500 200 T 180 CDS-06-431 DEPT-90 T 160 DEPT-135 140 120 100 لك -2 peaks u ppm 80 60 40 20 0You will check the outcome of reaction via infra-red spectroscopy: what are the diagnostic functional group peaks for the following compounds? You should also indicate the stretching motions responsible for absorbance. Dilantin benzil (unreacted starting material) Urea (unreacted starting material)QUESTION 5 The IR, 'H NMR and 1°C NMR spectra of compound P are shown below. The molecular formula for compound P is CsH;NO2. Identify three (3) important absorption peaks on the IR spectrum and indicate the bond and wavenumber associated with each peak. Elucidate the structure of compound P and assign each peak in the 'H and 13C NMR spectra. (Hint: The compound consists of more than one functional group). а. b.
- Lrdocaine USP (ATR) 95- 90 85 80 75 70 65 60 55 50 45 Lidocaine 40 4000 3500 3000 2500 2000 1500 1000 Wavenumbers (cm-1) %Transmittance IZ 3243.36 3021.30 2968.47 2921.13 2799.39 1661.66 1593.98 1491.43, 1449.061 422.12,384.681371.62 1291.03264.55 1206.99 1165.931119.56 1091.78072.77. 1034.33 970.79986.39 917.91, 885.23 763.76 703.94 615.01599.90Express the following absorbances (A) in terms of transmittance (T) and percent transmittance (%T) I. Note that: T- 10; %T-Tx100 A %T 1. 0.052 0.916 0.378 0.269 0.075 2345. What is the molar absorptivity when the percent transmittance is 56.2%, the pathlength is 2.00 cm, and the concentration is 0.0000748 M?