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What volume of a 40% v/v solution must be used to produce 500mL of 5% v/v solution?
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- If 400 mL of a 20% w/v solution were diluted to 2 L, what would be the finalpercentage strength?Calculate the volume of BSA stock that will be required to make the standard solutions needed to create the BSA standard curve. Be sure to show your work and include the volume of 0.02 M phosphate buffer required to reach a final volume of 1 mL. From a 2,000 μg/mL BSA stock, create 1 mL of each of the following stock solutions in 0.02 M phosphate buffer using individual microcentrifuge tubes: 50 μg/mL, 250 μg/mL, 500 μg/mL, 1,000 μg/mL, 1,250 μg/mL, 1,500 μg/mL. Be sure to properly label all the microcentrifuge tubes before creating the standards.a)Determine the amount of X (in gram) in order to prepare 100 mL of 50 mM stock solution of X. Show your work. (MW of X= 225) b)Determine the volume required (mL) from the stock solution X in (i) to prepare 500 mL of MS medium containing 5 mg/L X. Show your work.
- How many ml 2M NaOH would it take to neutralize 20 ml of 0.5M HCI?Using a P-1000 micropipette, you transfer 800 microliter of ddH2O to a beaker and measure its weight. It turns out to be only 780 milligram; what is the percent error of this micropipette?The material to be heated is in a long, cylindrical tube, and is viscous enough that any flow inside the tube can be ignored, i.e. the heating is by conduction alone). Also, consider the glass of the tube to be very thin so its thermal resistance can be ignored. The tube diameter is 1 cm and the properties of the material are the same those of water, given by 0.64 W/m*K, 1000 kg/m3, and 4180 J/kg*K, for thermal conductivity, density, and specific heat, respectively. The water bath temperature is 65 °C, and the initial temperature of the material is 27 °C. Without the agitation of the water bath, the heat transfer coefficient can be considered to be 128 W/m2*K. Considering the tube to be a long cylinder, how long does it take for the coldest point to heat up to a temperature corresponding to 99% of the total possible temperature increase? If the surrounding how water is agitated using a stirrer, leading to a considerably large surface heat transfer coefficient, how long does it…
- To make 100 ml 0.3% NP-40 solution for FISH post washing, how many ul 100% NP-40 solution is needed?It takes 175ml of 0.75 M H2SO4 to completely neutralize 285 ml of NaOH. What is the concentration of the NaOH solution?A 10-L stirred tank is used to cultivate mouse myeloma cells. Dry air is sparged from the bottom at 1.0 L/min to supply oxygen to the system. The gas composition at the outler is 16% oxygen, 3.5% CO2, 6.5% water vapor, and balanced nitrogen. The ambient pressure and temperature are 1 atm and 37 °C, respectively. The dissolved oxygen in the reactor is controlled at 20% of saturation with ambient air. What is the respiratory quotinent (RQ) (i.e., mole CO2 produced/mol O2 consumed)? Assume that the mole fraction of CO2 in the ambient air is negligible. The universal gas constant R = 0.082 L*atm/mol*K
- A 10-L stirred tank is used to cultivate mouse myeloma cells. Dry air is sparged from the bottom at 1.0 L/min to supply oxygen to the system. The gas composition at the outler is 16% oxygen, 3.5% CO2, 6.5% water vapor, and balanced nitrogen. The ambient pressure and temperature are 1 atm and 37 °C, respectively. The dissolved oxygen in the reactor is controlled at 20% of saturation with ambient air. a. Assuming the reactor is at a steady-state, what is the volumetric oxygen transfer coefficient (kLa)? b. The specific OUR of myeloma cells is 5*10-11 mmol/cell*h. What is the cell concentration? c. What is the respiratory quotinent (RQ) (i.e., mole CO2 produced/mol O2 consumed)? Assume that the mole fraction of CO2 in the ambient air is negligible. The universal gas constant R = 0.082 L*atm/mol*KA 5% dextrose in 1/2 normal saline (D5 1/2 NS) solution is commonly administered to patients needing post-operative IV fluids. How could you prepare 500 mL of this solution? Dextrose is 5% (m/v) and the NaCl is 0.45% (m/v).Plot a line weaver-burk graph for Km=6.30mM and Vmax=360uM/min when an experiment has 5 tubes with concentrations of substrates of 1.0mM, 10mM, 50mM, and 100mM.