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- briefly explain the principle behind this technique; affinity chromatography using a diagram to illustrate.In the spectrofluorometric analysis of quinine, which of the following statements/phrases can be considered a factor level? A. Concentration of 2 ppm of iodide B. Concentration of quenchers C. The use of iodide D. Use of QuenchersWhy can't we determine Vo, Ve and Vt for affinity chromatography?
- In UV/Visible spectrophotometer analysis for a multicomponent system, there are only two dyes used in the mixture, the two proportions should be totalled to 1.0. but on finding You got 0.6 in total. Explain the reasons for the difference.State the functions for each of these components used in the gel filtration chromatography using a column experiment. Bromophenol blue A mixture of albumin and bromophenol blue SpectrophotometerIn Gel filtration chromatography, when will you stop collecting eluents if sample is not colored?
- Three sugars (Sugar A, B and C) were applied to a line as part of the set-up of a Paper Chromatography experiment (method was as described in Practical Manual. After running the chromatography and staining, the following Retention factor (Rf) values were determined. Note that the distance travelled by the solvent was equal across the width of the chromatography paper. Sugar A, Rf = 0.23 Sugar B, Rf = 0.65 Sugar C, Rf = 0.27 What can be drawn from the results of this Paper Chromatography experiment? O Sugars A and C had a stronger interaction with the mobile phase, relative to Sugar B. O Sugar B had the strongest interaction with the stationary phase among Sugars A, B, and C. O Sugar B had the strongest interaction with the mobile phase among Sugars A, B, and C. O Distance travelled by Sugar B must have been shorter than that of Sugars A and C. O Sugar B must be a non-reducing sugar given its high Rf value.You are told to analyze the purity of your product with TLC. Which of the following TLC plates would indicate a pure product? Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. a A TLC plate where the product lane has one spot with an Rf value that exactly matches that of the starting material. b A TLC plate where the product lane has one spot which has a lower Rf value than the starting material. с A TLC plate where the product lane has two spots, one with a higher Rf value than the starting material and one with a lower Rf value. d A TLC plate where the product lane has one spot which has a higher Rf than the starting material.What are the mechanisms of samples separation work in Thin layer Chromatography? Please shortly write at your own words. Answer should be to the point (5-6 lines maximum).
- Identify the FALSE statement about visualization of thin layer chromatography results. A. The spots of colourless compounds are invisible to the eyes. B. These spots can be detęcted by putting the plate under ultraviolet light. C. These spots can be detected by placing the plate in a covered jar containing iodine crystals. D. None of the above. All are true statements.You have a stock solution of 40% hydrogen peroxide. You need to make several dilutions for a calibration curve. Calculate how much volume of the stock hydrogen peroxide you need to make 50 mL of 11% hydrogen peroxide.Choose the one answer that fits best. Which of the following statements regarding the proper procedure for using Micropipettes is NOT correct? O a. You cannot use a 2-20 µl micropipette to pipet 200 µl O b. To expel all the liquid from the tip, you have to press the eject button O c. To draw up solution, press and hold the plunger at the first stop before entering the solution O d. Micropipettes always require the use of a disposable plastic tip O e. While pipetting, micropipettes should always be held as straight as possible
