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- What is the function of buffer systems in living organisms and why are they important in the chemistry of the circulatory system?A stock solution was diluted in two steps. In a first step, 0.5 ml was diluted with 9.6 mL buffer. In a second step, 0.5 mL from the first step was diluted with 9.6 mL of buffer. The concentration of the mixture of the second step was determined to be 0.24 mg/mL. What was the concentration of the stock solution?How do you prepare a 100mL of 0.1 M phosphate buffer? From the anterior buffer, how do you make 100mL of 0.05 M? PLEASE GIVE THE MEASUREMENTS