Q: Why Do not add more than 2 drops of copper sulfate in Biuret reagent?
A: There are several biochemical tests to identify the presence of protein in a sample. There are tests…
Q: Why is Hydrogen peroxide solution so effective against anaerobes?
A: Hydrogen peroxide is a chemical compound that is acts as mild antiseptic. It is also an endogenous…
Q: What is the result of tube A in this Voges-proskauer test? What are the reagents of this test? What…
A: Voges-proskauer test is performed to differentiate between facultative anaerobic enteric bacteria.
Q: Why are antimicrobials inhibited in the presence of organic matter?
A: The different types of anti-microbials are anti-parasitic, anti-fungals, anti-bacterials, and…
Q: What is the importance of an antimicrobial removal device (ARD)?
A: The antimicrobial removal device (ARD) is a device that removes antibiotic residues from blood…
Q: Why is mannitol salt agar (MSA) considered both a selective and differential agar for staphylococci?
A: We’ll answer the first question since the exact one wasn’t specified. Please submit a new question…
Q: What is the logic behind testing for E. coli to detect fecalcontamination of water?
A: Bacteria are unicellular prokaryotic organism that maintains a definite shape since it contains the…
Q: What would be the expected out come of a Nitrate reduction test If the bacteria is a pathogenic…
A: Nitrate Reduction Test is used to determine the ability of an organism to reduce nitrate (NO3) to…
Q: What is the media used in Catalase test and its expected results?
A: Catalase is an enzyme that is found in the cell in most of the organism that protect the cell from…
Q: Why mightproteorhodopsin make a bacterium such as Pelagibacter morecompetitive in its habitat?
A: Proteorhodopsin(pRhodopsin) is a family of transmembrane proteins which is the most abundant retinal…
Q: Why do we test water for coliforms if they are not considered pathogenic?
A: Introduction Coliforms are gram-negative, non-sporulating and motile or immotile bacteria. They…
Q: Salmonella typhi is a facultative anaerobe. Explain why.
A: Salmonella is a genus of Gram-negative bacteria that are rod-shaped (bacillus) and belong to the…
Q: In the multiple fermentation tube technique does the absence of gas formation after 24 hours…
A: Coliform microbes are life forms that are available in the climate and in the defecation of all…
Q: why do microorganisms differ in their response to disinfectants
A: Microorganisms are the minute microscopic organism that is generally invisible to the human eye.…
Q: Explain why sulfa drugs prevent bacterial growth without harming the human host.
A: Antimicrobial drugs are those which destroy or inhibit the growth of microorganism. They have their…
Q: Identify the oxidase-negative, gram negative bacillus isolated from the blood cultures of a…
A: Gram-negative bacteria are those that do not retain the crystal violet stain after gram staining.…
Q: Which of the following medium:function classification is INCORRECT? Sabouraud Dextrose: selective…
A: These are the culture media used in Microbiology Lab to grow and recognize the different kind of…
Q: How alcohol is effective at contolling microbial growth?
A: Sterilization: Sterilization includes the complete wipeout of all microscopic life on a surface.…
Q: Which microorganism does not contain superoxide dismutase enzymes?
A: Superoxide dismutase (SOD) is an enzyme found altogether in living cells. An enzyme could be a…
Q: What is the antimicrobial removal device (ARD) blood culture system? Discuss its mechanism and its…
A: Microbes are present everywhere. Bacteria, viruses, and fungi are some of the types of…
Q: what is the cause of different species of psychrotrophic bacteria growing in the CTSA medium at 7…
A: They can grow at extreme low temperatures and lead to spoilage of food by modifying the compounds…
Q: Identify the microorganism/ group of microorganisms involved in the spoilage of Salmon pink…
A: Fermentation is a metabolic interaction that produces chemical changes in natural substrates through…
Q: Explain why it is important to use only a small amount of bacteria when preparing a smear
A:
Q: name the genus and species of 2 enteric bacteria that do not ferment lactose discuss why a mixed…
A: Hi! As you have posted multiple questions and not mentioned which one of them to be answered, I will…
Q: Discuss the result of sunflowers rhizofiltration of the pond near Chernobyl.
A: The most significant impact of human activity is pollution and habitat damage. Land-use changes may…
Q: Why does the oxidase test must be performed from blood agar or another medium without a fermentable…
A: Biochemical tests are used to identify microbial diversity based on variations in the biochemical…
Q: Assigned chemical agent: How does this agent specifically destroy microbial cells? Propose a…
A: There are few important points that should kept in mind : The bacteria treated with allows stain to…
Q: Using aseptic techniques, list the general procedure of inoculating a broth from a plate culture.
A: Aspetic techniques that you should follow during inoculating a broth from a plate culture are:…
Q: Which of the following tests can be used to distinguish between Enterobacter and Pseudomonas…
A: Introduction Small, single-celled organisms are known as bacteria. Nearly every place on Earth…
Q: selective agar media for Vibrio cholerae?
A: Vibrio cholerae is a facultative anaerobe. It is a comma-shaped bacteria. It is a species of…
Q: How does Pseudomonas aerogenosa contribute to the virulance of the pathogen?
A: Pseudomonas aeruginosa is a bacterium which is liable for serious infectious diseases, chronic…
Q: What is the most commonly used fixative in stool samples and why?
A: Fixative is a moist environment for transporting and preserving intestinal parasites in stool…
Q: You inoculate a species of bacteria on a MacConkey agar. It normally shows positive for growth and…
A: MacConkey Agar is a differential agar for Lactose fermentation while if no other Sugars are used,…
Q: Why is 15 minutes generally used for sterilization? Why is agar added to medium?
A: Sterilization is the process in which all life forms like microbes (fungi, bacteria, viruses,…
Q: What is the optimum growth temperature for most human pathogens? Explain.
A: Bacterial growth is the division of one bacterium into two daughter cells in a process called binary…
Q: What is bactericide exposure?
A: The term bactericide is related to bacteria. Bacteria are unicellular and prokaryotic organisms that…
Q: How can organisms develop resistance to chemical disinfectants?
A: Bacteria can develop resistance to disinfectants over time either by acquisition of exogenous mobile…
Q: Explain why it is important to wash the wells with PBS between the additions of each reagent?
A: ELISA is a enzyme linked immunosorbent eassay. It is a process to get the measurements of antigens…
Q: What microbe requires serum components to be added to the growth medium?
A: Introduction Microorganisms:- An organism that can be seen only through a microscope, They live in…
Q: selective agar media for gram-positive bacteria?
A: Phenyl Ethyl Alcohol Blood Agar is a selective media for gram positive bacteria.
Q: Other than the PYR test, what is another use of the PEP reagent?
A: PYR is a colorimetric method used to detect the presence of pyrolidonyl arylamidase activity in a…
Q: 1. Differentiate between defined and undefined culture media. 2. Define alpha, beta, and…
A: The culture medium is a solid, semi-solid or liquid substrate that is rich in nutrients and other…
Q: Draw a diagram to illustrate the procedure for the determination of the minimum inhibitory…
A: Microbes or microorganisms cannot be viewed with unaided eyes and require a microscope for their…
Q: What appropriate method should be used in controlling microbial growth in each of the following;…
A: The growth of microorganisms is controlled by means of physical agents and chemical agents. Physical…
Q: James inoculated a species of bacteria into KIA media and realized that it has a yellow butt and a…
A: Introduction KIA or the Kilger Iron Agar is a differential media that helps in distinguishing the…
Q: How is the CTSA (coconut tryptic soy agar plate) growth medium designed to meet the nutritional need…
A: TSA is a general-purpose plating medium that can be used to isolate, cultivate, and maintain a wide…
Q: equipments can be used to determine turbidity in the sample tubes in testing of antimicrobial act
A: The clarity of a liquid can be measured by its turbidity. We can define turbidity as the measure of…
Q: An unknown bacterium produces colorless colonies when inoculated onto an EMB plate. Predict what you…
A: EMB stands for Eosin Methylene Blue. It is a selective stain used for the growth of gram-negative…
Why does a precipitate form on Sierra’s medium when inoculated with a lipolytic organism? Discuss the principle behind this procedure.
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- In the multiple fermentation tube technique does the absence of gas formation after 24 hours constitute a negative presumptive test for coliform bacteria? Explain.Vibrio cholerae can be cultured on Thiosulfate-Citrate-Bile salts-Sucrose agar (TCBS). Enterobacteriaceae and gram-positive bacteria are inhibited on this agar. cholerae produces large yellow colonies while other Vibrio species produce blue, green, or blue-green colonies. Discuss the purpose of each media listed. Based on this information alone, what term(s) would best describe this agar? Selective Enrichment (enriched) Differential Reducing Selective and enriched Selective and differential Selective, differential, and enrichedExplain why sulfa drugs prevent bacterial growth without harming the human host.
- What appropriate method should be used in controlling microbial growth in each of the following? 1. Chicken serum 2. Fresh goat milk 3. Nutrient broth 4. Paracetamol 5. Inoculation loobAn unknown bacterium produces colorless colonies when inoculated onto an EMB plate. Predict what you would see if you inoculated that same unknown onto the Mannitol salt agar media?name the genus and species of 2 enteric bacteria that do not ferment lactose discuss why a mixed culture cannot be used to inoculate a differential media such as the tripple sugar iron agar test.
- You are transferring a bacterial culture from a tube of broth to a Petri dish containing trypticase soy agar. Please list the steps that are necessary to make the transfer successfully and with minimal risk. of contamination.Match the description to the correct medium This medium is enrichment for fastidious [Choose ] bacterial pathogens and differential based on hemolysis [Choose ] This medium is selective based on Tryptic Soy Agar, TSA salt/sodium chloride tolerance at 7.5 % Sabouraud Dextrose Agar, "Sab" agar weight/volume and differential based on mannitol fermentation Mannitol Salt Agar, "MSA" MacConkey Agar, "Mac" agar This medium is selective based on the ability to grow in bile salts and crystal violet and is EMB Eosin Methylene Blue Agar differetial based on lactose fermentation Blood Agar, "BA" this medium is all purpose and can grow a [Choose ] wide range of bacteria and fungiAfter a series of experiments, bacterial cultures were identified for disposal. How can these be properly decontaminated prior to disposal according to biosafety guidelines? METHOD:RATIONALE:
- Why is the effectiveness of an antimicrobial agent determined using an exponentially growing bacterial culture? Please explain.Using aseptic techniques, list the general procedure of inoculating a broth from a plate culture.If a bacterial isolate shows intermediate to moderate resistance to an antimicrobial, how might this drug still be successfully used in the treatment of this microbe?