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- Which letter represents the target site of furosemide (Lasix)? E- -BIn your own words, explain the term transposon tagging.What type of experiment can be performed to confirm that the C-terminal KDEL tetrapeptide is both necessary and sufficient in ER localization? (Necessary meaning that without KDEL, ER retrieval would not occur, and sufficient meaning that with KDEL, we would definitely see its effect (ER retrieval would occur))
- Explain what is TFIID ?A variety of organic chelating ligands have been synthesized to tightly coordinate radioactive metal cations to identify and treat malig- nancies by coupling the metal complex to a polypeptide linker attached to a monoclonal antibody that binds specifically to a cell surface re- ceptor. One such metal complex is illustrated in the diagram on the right. The radioactive Cu-64 cation is tightly coordinated by the che- lating ligand that is, in turn, conjugated to a peptide linker attached to a monoclonal antibody. (The antibody is not shown in this diagram.) When coupled to a specifically designed monoclonal antibody, the complex binds specifically to somatostatin receptors that are ex- pressed on the surface of neuroendocrine tumors. Subsequently the entire complex with the receptor is internalized, i.e., passed into the cytoplasm, where the radioactive metal cation kills the malignant cell. 1. CH3 HN NHNH HN NHNH HN Но НО NH HN S-S HN Но NH NH HN -NH HN- H2N OH NH ) Write the three…Electrophoretic Analysis of DNA via Agarose gel Electrophoresis determine the voltage used (how to determine the voltage? Hint: check on volt/cm).
- What is fadh2 used for?What is the utility of understanding the genetic basis of susceptibility to COVID-19 for medicine and public health? Can you think of a potential application of this knowledge?One of the causes of morbidity for the Zika Virus is Guillain-Barré Syndrome, an immune attack of nerve cells. One possible mechanism for this is the virus's interference with normal immune function. In HIV the virus uses the CD4 receptor to enter T-cells. To assess if this might be a possibility in Zika infections BLAST the CD4 Reference Sequence (NG 027688.1) against the Zika Virus (taxid:64320) choosing "blastn" under Program Selection (select all that apply): O None of the recovered sequences have identities of greater than 80%. O Multiple Zika virus isolates are observed to have E-values of less than 0.02. O High scoring BLAST hits are observed in Zika virus isolates from humans, mosquitoes, and monkeys. O The BLAST hits return a partial cds collected in Uganda in 1947 - isolate Uganda1947.
- What is the relationship between a PRR and a PAMP?In an analysis of five rII mutants, complementation testing yielded the following results:In a cotransformation experiment (see question 4 of More GeneticTIPS), DNA was isolated from a donor strain that was proA+ andstrC+ and sensitive to tetracycline. (The proA and strC genes conferthe ability to synthesize proline and confer streptomycin resistance,respectively.) A recipient strain is proA− and strC− and isresistant to tetracycline. After transformation, the bacteria werefirst streaked on a medium containing proline, streptomycin, andtetracycline. Colonies were then restreaked on a medium containingstreptomycin and tetracycline. (Note: Each type of medium hadcarbon and nitrogen sources for growth.) The following resultswere obtained:70 colonies grew on the medium containing proline, streptomycin,and tetracycline, but only 2 of these 70 colonies grew whenrestreaked on the medium containing streptomycin and tetracyclinebut lacking proline. If we assume the average size of the DNA fragments is 2 minutes,how far apart are these two genes?