Why Library screening is used ?

Q: When is fluorescence used in a clinical laboratory?

A: Fluorescence is a type of photoluminescence where light is absorbed by an excited molecule or atom…

Q: Describe the principle of "Whole Mount In Situ Hybridization" and explain why it is said to be a…

A: INTRODUCTION Whole mount In situ hybridization is a special technique that is used to see the…

Q: Why in the preparation of akaryotype analysis is the useof a substance like colchicineinteresting?

A: Karyotypes are prepared from mitotic cells that have been arrested in the metaphase or prometaphase…

Q: What is ORF scanning?

A: The open reading frame(ORF) in molecular genetics is a continuous stretch of codons that begins with…

Q: What is gel electrophoresis and why is it important? Write in detail.

A: Electrophoresis is the technique that is used to separate biomolecules that have a charge in them.…

Q: What is the use of light microscope in cytology and genetic studies

A: Cytology is the study of cell and cellular structures. Cytology and cytopathology are the main…

Q: Why is the wavelength of the light that is released in spectrophotometric analysis longer than the…

A: Some molecules absorb light at a particular wavelength and emit light in higher wavelength and this…

Q: Describe the process of doing a microarray.

A: A laboratory tool is used to measure the expression of thousands of genes at the same time and this…

Q: Why is it important count cells in research?

A: When seeding cells for later studies, determining immortalization or transformation, monitoring cell…

Q: Draw a alternative to the Kirby-Bauer procedure.

A: Introduction: The Kirby-Bauer procedure is also known as the disk diffusion method. It is a…

Q: What is FAME analysis?

A: FAME is an acronym which stands for Fatty Acid Methyl Ester which is produced by the process of…

Q: When we need to perform Southern, Western and Northern blotting? How they are different from one…

A: Blotting is the technique used to transfer the DNA, RNA, or protein sequence on a carrier membrane…

Q: Give three situations where negative staing procedure would be used?

A: Negative staining is a staining technique in which the background is stained whereas the specimen…

Q: What are the advantages and disadvantages of multitest systems for bacterial identification?

A: A multitest system is the combination of multiple biochemical tests useful in identification for the…

Q: what is the difference between presumptive and confirmational analysis?

A: There are certain tests which requires simple techniques while other require complex experimental…

Q: How does high throughput screening work?

A: High-throughput screening (HTS) is a automated process to test large numbers compounds (chemical or…

Q: What is high throughput screening used for?

A: High throughput screening:   It is a scientific method of drug discovery. With the use of robotics,…

Q: Explain the process of “blue-white” screening ?

A: Blue-white screening is a technique which allows proper detection of recombinant bacteria for…

Q: explain why gene mapping is important and how this technique can be used in disease investigation?

A: The Human Genome Project aimed at developing tools to identify new genes and their function. Genetic…

Q: Why use LC-MS for Bioanalysis?

A: Liquid chromatography -mass spectrometry is a powerful analytical tool used for separation,…

Q: The __________ program was developed by Dr. JohnDaugman for the analysis and verification of…

A: Iris The coloured ring around the pupil of the eye is called the iris. Control light levels inside…

Q: What do you mean by Southern Blotting technique?

A: Southern blotting is a laboratory technique that is widely used to detect a specific DNA sequence in…

Q: Explain ORF analysis.

A: Protein translocation is a process where proteins move between cellular components. It can occur…

Q: What are the potential advantages of promoting artificialphotosynthesis research?

A: Artificial photosynthesis is a process of an artificial method of copying the natural process of…

Q: Describe the agar-overlay technique

A: Biological techniques are methods or processes used to study living things. Methods, approaches,…

Q: Why some people resort to phenocopy?

A: Phenocopy is a phenomenon in which the phenotype (observable characteristics of an organism) of an…

Q: Where to start with microbial complete genome analysis?

A: Genomic analysis can be described as the process of recognizing, measuring, and comparing the…

Q: What type of probes must be used for super-resolutionmicroscopy and why?

A: The super-resolution microscopy involves a series of techniques that allow high-resolution images.…

Q: Define about library screening ?

A: Introduction :- The technique of identifying the clones carrying the gene of interest is known as…

Q: Explain the rationale and achievable goals behind using a genetic screen(like Heidelberg screen).…

A: Genetic screening such as Heidelberg screening is also called mutagenesis screening. This technique…

Q: What is the difference between low reolution and high resolution?

A: The capability of a microscope to identify details is referred to in microscopy as resolution. To…

Q: What can be the errors and limitations of Gel Electrophoresis practical? Explain in very much detail…

A: Errors and limitations of gel electrophoresis.

Q: "Specific Genes Can Be Recovered from a Library by Screening". Describe about this ?

A: The genes can be referred to as the basic unit that can be transferred from parents to the next…

Q: blue-white colony selection method in the recombinant colony screening

A: Blue - white screening for selection recombinat colony :- Blue - white screening is a rapid and…

Q: Advantages and disadvantages of using nucleic acid detection assay in diagnostic virology

A: Nucleic acid detection assay is a method which is used for the detection of specific sequence of…

Q: Can gel electrophoresis be a quantitative technique? How so?

A: DNA, proteins, RNA are biomolecules which are very crucial in a cell. Many different techniques are…

Q: Calculate the scan time for a GRE with TR = 30 msec, NEX = 2, Ny = 256, for (a) a single slice and…

A: The gradient-echo (GRE) pulse sequence is also known as gradient recalled echo. The main purpose…

Q: Why do you need to add the enzyme just before starting UV acquisition

A: Lambert-Beer Law: This law states that the value of the absorbance we get, is directly dependent…

Q: Which requires more effort either screening or selection

A: Successful evolutionary bio engineering requires a high throughput screening or selection method,…

Q: What is the term ‘Eidetic image' refers to?

A: In the literature of science, the term eidetic image comes close to photographic memory.

Q: Define centrifugation and aliquoting.

A: Centrifuge is a device that is used for the separation of different components of a fluid with the…

Q: If a non-science person asks you what gel electrophoresis is, how are you going to explain the…

A: Gel electrophoresis concept explanation to a non- science person.

Q: purpose of coding.

A: Medical coding consists of the explanation of the diseases, trauma, and healthcare techniques from…

Q: What are the limitation of retrospective analysis?

A: There are different types of epidemiological studies such as, 1. Observational studies: It has two…

Q: What is probe? Why it is used in the library screening ?

A: Biotechnology is the practice of using living organisms such as animals, plants, microbes, and…

Q: How Gel electrophoresis is used ?

A: DNA profiling is the process of determining an individual's unique DNA pattern. DNA profiling can…