Q: What is the purpose of the alcohol (OH) in the gram staining process?
A: Usually, microbes are colorless, so it’s very difficult to see them even after magnifying under…
Q: Is it possible to perform double or triple staining using fluorescent dyes? Explain
A: For both membrane and soluble proteins, fluorescent dyes are increasingly being utilized to monitor…
Q: hello, can you draw the following on endospore staining in 1000x objective? thank you. - 1 day…
A: Bacillus is a genus which is a endospore forming, rod shaped, gram positive bacteria.
Q: What kind of stain would a pathologist use to visualise a chrondrosarcoma under the microscope? why…
A: Staining is a method for enhancing contrast in material, usually on a tiny scale. Biological…
Q: Negative Stain Name the bacterium used in negative stain Describe morphology Name the disease…
A:
Q: After conducting the Gram staining procedures and you observe nothing (you didn’t see anything) in…
A: Introduction Gram staining, commonly known as Gram's method, is a staining technique that is used to…
Q: If the iodine step in a Gram-staining procedure was omitted, what color would you expect…
A: Gram staining is a technique which is used to identify the bacterial cell type, whether it is gram…
Q: What is gram staining and its purpose? Why is it only applicable in bacteria?
A: Introduction: Differential staining methods like Gram staining, acid-fast staining, endospore…
Q: What are the pros and cons of using the TCE stain-free method versus a traditional gel staining…
A: PAGE (polyacrylamide gel electrophoresis) is a well-known method for separating, detecting, and…
Q: Is the flagellar stain a Positive or Negative Simple Stain protocol?
A: Staining is a technique that is used to enhance contrast in samples, generally at the microscopic…
Q: Is heat fixation used on bacterial smears that will undergo a capsule stain or flagella stain…
A: In capsule staining procedure “we do not heat fix and rinse the smear with water” as heat and water…
Q: What color would gram positive cells appear if ghr safranin staining step was left out of staining…
A: Gram staining is a technique used for differentiating the bacteria based on their cell wall…
Q: Are the structural differences between the cell walls of Gram-positive and Gram-negative bacteria…
A: The gram-positive and negative cells differ in their cell wall composition.
Q: If you were working with an unlabeled simple stained smear, would you be able to identify the…
A: Staining is used to colour biological specimen such as cell to identify them. Simple staining…
Q: Which of the microscope objectives is most satisfactory for studying bacteria? Why?
A: For studying bacteria , under the microscope , 100X objective oil immersion lens is generally used…
Q: Why are bacterial cells generally stained for microscopic viewing?
A: Staining bacterial cells prior to microscopic viewing is a routine process in most of microbiology…
Q: How do gram-positive and gram-negative bacteria differ in cellular structure, and how does this…
A: Gram staining is a most important technique in the microbiology to differentiate the group of…
Q: A differential stain can: separate bacteria into groups by cytoplasm composition OA. separate…
A: The small parts of the microorganisms are hard to differentiate as the colors under the microscope…
Q: What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and…
A: What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and…
Q: You are performing a Gram stain on Gram-negative bacteria, and you stop after the addition of the…
A: Gram negative bacteria: these bacteria are enclosed in hard capsule and don't retain the crystal…
Q: What technique is used to stain this bacterium? What kind of stain is used? What type of…
A: The staining is done to observe the bacteria under the microscope, there are different type of…
Q: How is true microbial motility distinguished from other kinds of motion like Brownian movement that…
A: Q. How is true microbial motility distinguished from other kinds of motion like Brownian movement…
Q: What is the accepted theory regarding the mechanism of the Gram stain?
A: Bacteria on the basis of the structural organization of their cell wall are of two types:…
Q: Based from the results obtained from Gram staining, explain why bacterial cells retained or changed…
A: Staining is a technique that is used to enhance the contrast of the microscopic image through the…
Q: What is the name of bacteria for each gram staining and identify the shape and the arrangement?
A: Gram staining is the method of identification between the gram positive and gram negative bacteria.…
Q: Why do cheek cells stain red with the gram stain? What is the big red circle in the middle of the…
A: Bacteria that have a thick layer of a substance called "peptidoglycan" in their cell wall will get a…
Q: How would the slide appear if the bacteria were stained with safranin, a basic red dye?
A: The appearance of bacteria, when stained with safranin, is to be determined.
Q: why the Gram stain can differentiate between Gram positive and Gramnegative cells?
A: *Gram staining is a technique used to differentiate two groups of bacteria into gram positive and…
Q: Why are smears of capsulated bacteria not heat-fixed before staining as opposed to Gram staining?
A: Gram staining is a differential staining technique developed by Danish bateriologist Hans Christian…
Q: Illustrated Diagram: Make an illustrated diagram of a stepwise procedure for the performance of…
A: The staining is done to enhance the visualization of cell and certain cellular component under a…
Q: what is the function of each one of the Gram-stain reagents?
A: Gram staining is the technique used to classify the bacterial species into broad groups based on the…
Q: In the Gram staining procedure, how do the dyes able to distinguish Gram positive from Gram negative…
A: Gram staining, commonly known as Gram's technique, is a staining technique that is utlised to divide…
Q: 400x 1000x What is the shape of bacteria under a microscope, what color is there, and is there Gram…
A: INTRODUCTION Gram staining is a differential staining used to differentiate bacteria into gram…
Q: What is the primary stain for the Ziehl-Neelson acid fast stain? What color is this stain? What…
A: In microbiology, the technique that is used to enhance the contrast in samples, at the microscopic…
Q: Which of the following is/are true regarding the acid-fast stain? (There may be more than one…
A: Answer First option Non-acid-fast microbes appear blue in a completed acid-fast stain.
Q: the chemicals used in the gram staining method?
A: The bacteriologist Hans Christian Gram was developed gram staining method in 1884. the Gram staining…
Q: What color are Gram negative cells after decolorizing with ethanol? What is the best age for your…
A: Since you have posted a question with multiple sub-parts, we will solve the first three sub-parts…
Q: what is the theory about the mechanism of the gram-stain reaction
A: Introduction :- A Gram stain test looks for bacteria in areas where an infection is thought to have…
Q: what would be the medical significance of finding a positive endospore stain result from bacilli…
A: Endospores are thick walled structures found in the genus Bacillus and Clostridium.
Q: What is the formula used to describe the resolution of a microscope lens, and what are the…
A: The term resolution is used in microscopy to characterize a microscope's capacity to identify…
Q: How can colony morphology be used in the identification of bacterial species? What are the…
A: NOTE: AS PER OUR GUIDELINES, WE ARE ONLY SUPPOSED TO ANSWER THE FIRST THREE QUESTIONS, PLEASE…
Q: Which bacterial type ( Gram positive or Gram negative) is more sensitive to the antibiotic…
A: Penicillin is the group of antibiotics which were originally derived from the penicillium molds. It…
Q: What is the theory about the mechanism of the Gram-stain reaction? provide in paragpraph
A:
Q: how is gram staining useful in differentiating neisseria meningitidis, streptococcus pneumoniae, and…
A: Hi, Thanks For Your Question. Answer : Gram staining helps in differentiation of various bacteria…
Q: Endospores can be seen as refractile structures in unstained cells and as colorless areas in…
A: Endospores are dormant, non-reproductive structures formed by certain bacteria during unfavorable…
Q: What color wouod gram negative cells appear if the decolorizing step was left out of staining…
A: Gram staining method: This technique is useful in identifying two groups of bacteria based on the…
Q: Are there any chemical differences between the cell walls of gram-positive and negative bacteria…
A: Bacteria are the prokaryotes which fall under Kingdom Monera. They can be found everywhere. They…
Q: What are the principle and basic concepts of GRAM STAIN?
A: Gram staining is among the most important microbiology staining procedures. It was first invented in…
Q: What is the principle of image formation on transmission election microscope and scanning electron…
A: The general biological microscope helps in the visualisation of the image of a particular sample…
Q: What is the difference between simple and differential stains? Give an example of a differential…
A: Bacteria are microorganisms that are colorless for most of the part and in order to study their…
- Would lysozyme or penicillin affect pneumoniae?
- What factors normally limit the resolving power of a light microscope?
- In the
Gram stain procedure, which step is most time-sensitive?
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
- Why is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?Is the gram stain significant to microorganisms other than bacteria? explainDescribe the procedure for performing a Gram stain. What is the procedure? How does each component work? (ie. What does the mordant do?)
- Bacillus subtilis (Gram-positive) and Pseudomonas aeroginosa (Gram-negative) bacteria are heat-fixed on slide and subjected to Gram staining. What do you observe under the microscope when the following steps are skipped? Please explain.a. Staining with safraninb. Staining with Gram iodinec. DecolorizationIs there a relationship between Gram staining and sensitivity to various antibacterial compounds? Please explain.How can colony morphology be used in the identification of bacterial species? What are the environmental factors that affect the growth of bacteria? What is agar (or agar-agar)? Is this different from nutrient agar? Why? How is xylol used to clean the lens of a microscope? What may happen if xylol is constantly used for this purpose? For what purpose would you adjust each of the following microscope components during a microscopy exercise? iris diaphragm coarse adjustment knob fine adjustment knob condenser mechanical stage control
- Can the gram stain be observed using a form of microscopy other than light microscopy? Why or whynot?What staining technique is used here? What is the primary stain, decolorizing agent, and counter stain of this technique? Do we heat fix in here? Describe the organism with the blue color. Describe the organism with the bright pink color. Name the genus of bacteria with bright pink.Why is mordant necessary for the staining of bacterial flagella? Answer in not more than 3 sentences.
- Why are basic dyes more effective for bacterial staining than acidic dyes?a. Identify the cell shape, cell arrangement, and Gram reaction of the Unknown microbe in the image below. b. Suppose the corresponding Gram negative control were purple. Explain what step(s) of the Gram stain you would adjust and how you would adjust them to ensure the accuracy of your Unknown resultsWhat are the principle and basic concepts of GRAM STAIN? (please explain it thoroughly in a lengthy but clear explanation, thank you so much.)